Project description:ATHB8 is a member of HD-ZIP III family transcription factors and has a important role in vascular development. However, how ATHB8 regulates vascular development is still a mystery. Here, we identified specific genes downstream of ATHB8-GFP with transformed suspension culture cells in microarray experiments.
Project description:VND1 is a member of NAC-type family transcription factors and has a important role in xylem differentiation. However, how VND1 regulates vascular differentiation is not well known. Here, we identified specific genes downstream of VND1 with transformed suspension culture cells in microarray experiments.
Project description:Plant vascular tissues are essential for the existence of land plants. Many studies have revealed the process underlying the development of vascular tissues. However, the initiation of vascular development is still a mystery. LONESOME HIGHWAY (LHW), which encodes a bHLH transcription factor, is expressed in the initial step of vascular development in roots. LHW and TMO5 LIKE1 (T5L1) interact each other and function as a heterodimer. Here, we identified specific genes downstream of LHW-T5L1 with transformed suspension culture cells in microarray experiments.
Project description:Ten-day old seedlings of Arabidopsis wildtype Col-0, and SsE1-overexpressing lines SsE1-GFP-5 and SsE1-GFP-8 were collected for total RNA extraction. Three biological replicates were prepared for each genotype.
Project description:Plant vascular tissues are essential for the existence of land plants. Many studies have revealed the process underlying the development of vascular tissues. However, the initiation of vascular development is still a mystery. LONESOME HIGHWAY (LHW), which encodes a bHLH transcription factor, is expressed in the initial step of vascular development in roots. LHW and TMO5 LIKE1 (T5L1) interact each other and function as a heterodimer. Here, we identified specific genes downstream of LHW-T5L1 with transformed suspension culture cells in microarray experiments. To identify genes downstream of LHW-T5L1, we collected three samples at 0h and 12h with or without estrogen with triplicates.
Project description:Xylem consists of three types of cells: vessel cells, also referred to as tracheary elements (TEs), parenchyma cells, and fiber cells. TE differentiation includes two essential processes, programmed cell death (PCD) and secondary cell wall formation. These two processes are tightly coupled. However, little is known about the molecular mechanism of their gene regulation. Here, we show that VASCULAR-RELATED NAC-DOMAIN 6 (VND6), a master regulator of TEs, regulates these processes in a coordinated manner. We first identified specific genes downstream of VND6 by comparing them with those of SECONDARY WALL-ASSOCIATES NAC DOMAIN PROTEIN1 (SND1), a master regulator of xylem fiber cells, with transformed suspension culture cells in microarray experiments.
Project description:In order to identify the effects of TFEB overexpression on the hela cells transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the hela TFEB stable clones Transcriptome analysis of hela stable clones overexpressing TFEB-GFP