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ABSTRACT: Effects of Bacillus subtilis and Lactobacillus buchneri on fermentation, aerobic stability, and microbial community of whole plant corn silage

PROVIDER: PRJNA890780 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21918800_1.fastq.gz	Fastqsanger.gz
	SRR21918800_2.fastq.gz	Fastqsanger.gz
	SRR21918801_1.fastq.gz	Fastqsanger.gz
	SRR21918801_2.fastq.gz	Fastqsanger.gz
	SRR21918802_1.fastq.gz	Fastqsanger.gz

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Effects and mechanism of Neolamarckia cadamba essential oil on the aerobic stability of whole-plant corn silage

Project description:The purpose of this study was to explore the mechanism of aerobic decay of whole-plant corn silage and the effect of Neolamarckia cadamba essential oil on aerobic stability of whole-plant corn silage. Firstly, the dynamic changes of temperature, microbial community and metabolite content after aerobic exposure of whole-plant corn silage were determined, and the main microbial species and mechanism leading to aerobic spoilage of whole-plant corn silage were analyzed. The N. cadamba essential oil was extracted from fresh N. cadamba leaves by steam distillation, and the minimal inhibitory concentration, antibacterial stability and bacteriostatic mechanism of N. cadamba essential oil against undesirable microorganisms in whole-plant corn silage were determined. According to the minimum inhibitory concentration of N. cadamba essential oil on undesirable microorganisms in silage, N. cadamba essential oil was added to whole-plant corn silage to explore the effect of N. cadamba essential oil on the aerobic stability of whole-plant corn silage.

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Differential Analysis of L. buchneri Transcriptomes under anaerobic and aerobic conditions

Project description:The Lactobacillus buchneri CD034 strain, known to improve the ensiling process of green fodder and the quality of the silage itself was transcriptionally analyzed by sequencing of transcriptomes isolated under anaerobic vs. aerobic conditions. L. buchneri CD034 was first cultivated under anaerobic conditions and then shifted to aerobic conditions by aeration with 21% oxygen. Cultivations already showed that oxygen was consumed by L. buchneri CD034 after aeration of the culture while growth of L. buchneri CD034 was still observed. RNA sequencing data revealed that irrespective of the oxygen status of the culture, the most abundantly transcribed genes are required for basic cell functions such as protein biosynthesis, energy metabolism and lactic acid fermentation. Under aerobic conditions, 283 genes were found to be transcriptionally up-regulated while 198 genes were found to be down-regulated (p-value < 0.01). Up-regulated genes i. a. play a role in oxygen consumption via oxidation of pyruvate or lactate (pox, lctO). Additionally, genes encoding proteins required for decomposition of reactive oxygen species (ROS) such as glutathione reductase or NADH peroxidase were also found to be up-regulated. Genes related to pH homeostasis and redox potential balance were found to be down-regulated under aerobic conditions. Overall, genes required for lactic acid fermentation were hardly affected by the growth conditions applied. Genes identified to be differentially transcribed depending on the aeration status of the culture are suggested to specify the favorable performance of the strain in silage formation.

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Project description:Dynamics of bacterial and fungal communities during aerobic exposure in whole-plant corn silage inoculated with Lactobacillus buchneri or Bacillus licheniformis

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Project description:Spineless cactus silage fermentation and aerobic stability

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Project description:Effects of Bacillus coagulans and Lactobacillus plantarum on the Fermentation Quality, Aerobic Stability and Microbial Community of Triticale Silage

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Project description:Effects and Mechanism of Neolamarckia cadamba Essential Oil on Aerobic Stability of Whole-plant Corn Silage

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