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Characterizing role of striated-muscle specific ribosomal protein L3-like (Rpl3L) in mouse hearts

ABSTRACT: Characterizing role of striated-muscle specific ribosomal protein L3-like (Rpl3L) in mouse hearts

PROVIDER: PRJNA891061 | ENA |

REPOSITORIES: ENA

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Characterizing role of striated-muscle specific ribosomal protein L3-like (Rpl3L) in mouse hearts

Project description:Overall goal: To elucidate the function served by Rpl3L in heart. Purpose of analysis: To determine effects of Rpl3L-deficiency on translational mRNA profile in heart. Experimental structure: Polysomes and monosomes were fractionated from cardiac ventricles and associated RNA extracted for library generation and differential gene expression analyses between wild-type and Rpl3L-null samples, using standard RNAseq pipeline.

2023-02-10 | GSE215869 | GEO

Defining the effects of Rpl3L-deficiency on transcriptional profiles of mouse hearts

Project description:The ribosomal protein L3-like (RPL3L) is a striated muscle-specific ribosomal protein, and mutations in human RPL3L are linked with childhood cardiomyopathy and age-related atrial fibrillation. However, the function served by this protein, a paralogue of the ubiquitously expressed RPL3 protein, remains poorly characterized in both heart and skeletal muscle. To address this, null mutant mice with the Rpl3L gene deleted were generated and studied. The purpose of this microarray analyses was to compare transcriptional profiles of the null mutant hearts (lacking RPL3L) with wild-type control hearts (expressing RpL3L). We used microarrays to elucidate effects of Rpl3L deficiency on transcriptional profile of mouse hearts

2023-02-10 | GSE216168 | GEO

Nano-TRAP Input and IP sequencing of WT and Rpl3l-/- mouse hearts

Project description:RPL3L is a ribosomal protein expressed exclusively in adult straited muscle tissues, and a paralogue of the ubiquitously expressed RPL3. Here, we are looking into the effects of Rpl3l knockout on translation in adult mice hearts.

2022-05-16 | GSE189872 | GEO

Ribo-Seq and RNA-seq sequencing of WT and Rpl3l-/- mouse hearts

2022-05-16 | GSE189854 | GEO

RPL3L-containing ribosomes determine translation elongation dynamics required for cardiac function

Project description:Although several ribosomal protein (RP) paralogs are expressed in a tissue-specific manner, how these proteins affect translation and why they are required only in certain tissues have remained unclear. Here we show that RPL3L, a paralog of RPL3 specifically expressed in heart and skeletal muscle, influences translation elongation dynamics. Deficiency of RPL3L-containing ribosomes (RPL3L-ribosomes) in RPL3L knockout male mice resulted in impaired cardiac contractility. Ribosome occupancy at mRNA codons was found to be altered in the RPL3L-deficient heart, and the changes were negatively correlated with those observed in myoblasts overexpressing RPL3L. RPL3L-ribosomes were less prone to collisions compared with RPL3-containing canonical ribosomes. Although the loss of RPL3L-ribosomes altered translation elongation dynamics for the entire transcriptome, its effects were most pronounced for transcripts related to cardiac muscle contraction and dilated cardiomyopathy, with the abundance of the encoded proteins being correspondingly decreased. Our results provide new insight into the mechanisms and physiological relevance of tissue-specific translational regulation.

2023-03-15 | GSE203072 | GEO

The role of Rpl3l in translation in cardiomyocytes

Project description:Ribosome immunoprecipitates of cardiomyocytes isolated from Rpl3l-/- and Rpl3l+/+ mouse hearts were analysed using mass spectrometry.

2023-03-08 | PXD026985 | Pride

Achromobacter sp. L3 strain:L3

Project description:Achromobacter sp. L3 strain:L3 Genome sequencing

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Staphylococcus epidermidis L3 strain:L3

Project description:Staphylococcus epidermidis L3 strain:L3 Genome sequencing

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Mammalian heart RPL3L

Project description:RPL3L-containing ribosomes modulate mitochondrial activity in the mammalian heart.

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Striated-for-smooth muscle replacement in the developing mouse esophagus

Project description:The esophagus is a muscular tube which transports swallowed content from the oral cavity and the pharynx to the stomach. Early in mouse development, an entire layer of the esophagus, the muscularis externa, consists of differentiated smooth muscle cells. Starting shortly after mid-gestation till about two weeks after birth, the muscularis externa almost entirely consists of striated muscle. This proximal-to-distal replacement of smooth muscle by the striated muscle depends on a number of factors. To identify the nature of the hypothetical “proximal” (mainly striated muscle originating) and “distal” (mainly smooth muscle originating) signals that govern the striated-for-smooth muscle replacement, we compared the esophagus of Myf5:MyoD null fetuses completely lacking striated muscle to the normal control using cDNA microarray analysis, followed by a comprehensive databases search. Here we provide an insight into the nature of “proximal” and “distal” signals that govern the striated-for-smooth muscle replacement in the esophagus.

2018-11-01 | GSE122017 | GEO

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