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Gene expression profile at single cell level of peripheral blood B cells from a CID patient (P) caused by IRF4 p.T95R heterozygous mutation and an age-matched healthy control (HC)

ABSTRACT: Gene expression profile at single cell level of peripheral blood B cells from a CID patient (P) caused by IRF4 p.T95R heterozygous mutation and an age-matched healthy control (HC)

PROVIDER: PRJNA891323 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21934223_1.fastq.gz	Fastqsanger.gz
	SRR21934223_2.fastq.gz	Fastqsanger.gz
	SRR21934224_1.fastq.gz	Fastqsanger.gz
	SRR21934224_2.fastq.gz	Fastqsanger.gz
	SRR21934225_1.fastq.gz	Fastqsanger.gz

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Project description:The centromere-specific Histone H3-variant CENH3 (also known as CENP-A) is considered to be an epigenetic mark for establishment and propagation of centromere identity. Pulse-induction of CENH3 (Drosophila CID) in Schneider S2 cells incorporates into noncentromeric regions and generates CID islands that resist clearing from chromosome arms for multiple cell generations. We demonstrate that CID islands represent functional ectopic kinetochores, which are non-randomly distributed on the chromosome and display a preferential localization near telomeres and pericentric heterochromatin in transcriptionally silent, intergenic chromatin domains. Although overexpression of heterochromatin protein 1 (HP1) or increasing Histone acetylation interferes with CID islands formation on a global scale, induction of a locally defined region of synthetic heterochromatin by targeting HP1-LacI fusions to stably integrated Lac Operator arrays produces a proximal hotspot for CID islands formation. These data suggest that the characteristics of regions bordering heterochromatin promote de novo kinetochore assembly and thereby contribute to centromere identity. ArrayExpress Release Date: 2011-07-15 Person Roles: submitter Person Last Name: Diehl Person First Name: Sarah Person Mid Initials: Person Email: diehl@immunbio.mpg.de Person Phone: (+49) 761 5108 795 Person Address: Stuebeweg 51, 79108 Freiburg im Breisgau, Germany Person Affiliation: Max-Planck-Institute for Immunobiology and Epigenetics Person Roles: investigator Person Last Name: Heun Person First Name: Patrick Person Mid Initials: Person Email: heun@immunbio.mpg.de Person Phone: (+49) 761 5108 717 Person Address: Stuebeweg 51, 79108 Freiburg im Breisgau, Germany Person Affiliation: Max-Planck-Institute for Immunobiology and Epigenetics Publication Title: Heterochromatin boundaries are hotspots for de novo kinetochore formation. Publication Author List: Agata Olszak, Dominic van Essen, Antonio J. Pereira, Sarah Diehl, Thomas Manke, Helder Maiato, Simona Saccani and Patrick Heun

Dataset Information

Gene expression profile at single cell level of peripheral blood B cells from a CID patient (P) caused by IRF4 p.T95R heterozygous mutation and an age-matched healthy control (HC)

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