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Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [TOMO-seq]

ABSTRACT: Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [TOMO-seq]

PROVIDER: PRJNA891645 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21946553_1.fastq.gz	Fastqsanger.gz
	SRR21946553_2.fastq.gz	Fastqsanger.gz
	SRR21946554_1.fastq.gz	Fastqsanger.gz
	SRR21946554_2.fastq.gz	Fastqsanger.gz

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Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [TOMO-seq]

Project description:Integrated in vitro models of human organogenesis are needed to elucidate the multi-systemic events underlying development and disease. We report the generation of human trunk-like structures that model the co-morphogenesis, patterning, and differentiation of the human spine and spinal cord. We identified differentiation conditions for human pluripotent stem cells favoring the formation of an embryo-like extending antero-posterior (AP) axis. Single cell and spatial transcriptomics show that somitic and spinal cord differentiation trajectories organize along this axis and can self-assemble into neural tubes surrounded by somites upon extracellular matrix addition. Morphogenesis is coupled with AP patterning mechanisms which results, at later stages of organogenesis, in in vivo-like arrays of neural subtypes along a neural tube surrounded by spine and muscle progenitors contacted by neuronal projections. This integrated system of trunk development indicates that in vivo-like multi-tissue morphogenesis and topographic organization of terminal cell types can be achieved in human organoids, opening windows for the development of more complex models of organogenesis.

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Project description:Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis.

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Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis.

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