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Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [TOMO-seq]

ABSTRACT: Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [TOMO-seq]

PROVIDER: PRJNA891645 | ENA |

REPOSITORIES: ENA

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Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [TOMO-seq]

Project description:Integrated in vitro models of human organogenesis are needed to elucidate the multi-systemic events underlying development and disease. We report the generation of human trunk-like structures that model the co-morphogenesis, patterning, and differentiation of the human spine and spinal cord. We identified differentiation conditions for human pluripotent stem cells favoring the formation of an embryo-like extending antero-posterior (AP) axis. Single cell and spatial transcriptomics show that somitic and spinal cord differentiation trajectories organize along this axis and can self-assemble into neural tubes surrounded by somites upon extracellular matrix addition. Morphogenesis is coupled with AP patterning mechanisms which results, at later stages of organogenesis, in in vivo-like arrays of neural subtypes along a neural tube surrounded by spine and muscle progenitors contacted by neuronal projections. This integrated system of trunk development indicates that in vivo-like multi-tissue morphogenesis and topographic organization of terminal cell types can be achieved in human organoids, opening windows for the development of more complex models of organogenesis.

2023-08-31 | GSE215982 | GEO

Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis.

Project description:Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis.

| PRJNA891638 | ENA

Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [scRNA-seq]

Project description:Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [scRNA-seq]

| PRJNA891643 | ENA

Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis [scRNA-seq]

2023-08-31 | GSE215981 | GEO

Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-08-31 | GSE215983 | GEO

Self-organizing human trunk 3D neuromuscular organoids

Project description:Self-organizing human trunk 3D neuromuscular organoids

| PRJNA527260 | ENA

Tomo-Seq of Dugeisa japonica along AP axis

Project description:In order to reveal temporal gene expression pattern in intact Dugesia japonica, we conducted high-thoriughput version of Tomo-Seq along the AP, DV, and LR axis.

2024-09-25 | GSE207273 | GEO

Tomo-seq of Pristionchus

Project description:Tomo-seq of Pristionchus pacificus nematodes

| PRJEB37439 | ENA

Self-organizing human trunk 3D neuromuscular organoids

Project description:Neuromuscular networks assemble during early human embryonic development and are essential for the control of body movement. Previous neuromuscular junction modeling efforts using human pluripotent stem cells (hPSCs) generated either spinal cord neurons or skeletal muscles in monolayer culture. Here, we use hPSC-derived axial stem cells, the building blocks of the posterior body, to simultaneously generate spinal cord neurons and skeletal muscle cells that self-organize to generate human neuromuscular organoids (NMOs) that can be maintained in 3D for several months. Single-cell RNA-sequencing of individual organoids revealed reproducibility across experiments and enabled the tracking of the neural and mesodermal differentiation trajectories as organoids developed and matured. NMOs contain functional neuromuscular junctions supported by terminal Schwann cells. They contract and develop central pattern generator-like neuronal circuits. Finally, we successfully use NMOs to recapitulate key aspects of myasthenia gravis pathology, thus highlighting the significant potential of NMOs for modeling neuromuscular diseases in the future.

2020-01-20 | GSE128357 | GEO

Transcription profiling of livers from three-spine sticklebacks to test a custom Agilent three-spine stickleback array

Project description:Liver tissue from three-spine stickleback individuals from 3 populations were tested on arrays to determine array suitability for transcriptomics experiments.

2009-08-21 | E-MEXP-2304 | biostudies-arrayexpress

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