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Stress responses elicited by glucose withdrawal in Aspergillus fumigatus

ABSTRACT: Stress responses elicited by glucose withdrawal in Aspergillus fumigatus

PROVIDER: PRJNA891691 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21948700.fastq.gz	Fastqsanger.gz
	SRR21948701.fastq.gz	Fastqsanger.gz
	SRR21948702.fastq.gz	Fastqsanger.gz
	SRR21948703.fastq.gz	Fastqsanger.gz
	SRR21948704.fastq.gz	Fastqsanger.gz

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Stress responses elicited by glucose withdrawal in Aspergillus fumigatus

Project description:Glucose is a widely used carbon source in laboratory practice to culture Aspergillus fumigatus, however, glucose availability is often low in its “natural habitats” including the human body. We used a physiological–transcriptomical approach to reveal differences between A. fumigatus Af293 cultures incubated on glucose, glucose and peptone, peptone (carbon limitation), or without any carbon source (carbon starvation). Autolytic cell wall degradation was upregulated by both carbon starvation and limitation. The importance of autolytic cell wall degradation in adaptation to carbon stress was also highlighted by approximately 12.4% of the A. fumigatus genomes harbor duplication of genes involved in N-acetyl glucosamine utilization. Glucose withdrawal increased redox imbalance, altered both the transcription of antioxidative enzyme genes and oxidative stress tolerance, downregulated iron acquisition, but upregulated heme protein genes. Transcriptional activity of the Gliotoxin cluster was low in all experiments, while the Fumagillin cluster showed substantial activity both on glucose and under carbon starvation, and the Hexadehydro-astechrome cluster only on glucose. We concluded that glucose withdrawal substantially modified the physiology of A. fumigatus including processes that contribute to virulence. This may explain the challenge of predicting the in vivo behavior of A. fumigatus based on data from glucose rich cultures.

2022-12-09 | GSE216000 | GEO

Stress Responses Elicited by Glucose Withdrawal in <i>Aspergillus fumigatus</i>.

Project description:Glucose is a widely used carbon source in laboratory practice to culture Aspergillus&nbsp;fumigatus, however, glucose availability is often low in its "natural habitats", including the human body. We used a physiological-transcriptomical approach to reveal differences between A. fumigatus Af293 cultures incubated on glucose, glucose and peptone, peptone (carbon limitation), or without any carbon source (carbon starvation). Autolytic cell wall degradation was upregulated by both carbon starvation and limitation. The importance of autolytic cell wall degradation in the adaptation to carbon stress was also highlighted by approximately 12.4% of the A. fumigatus genomes harboring duplication of genes involved in N-acetyl glucosamine utilization. Glucose withdrawal increased redox imbalance, altered both the transcription of antioxidative enzyme genes and oxidative stress tolerance, and downregulated iron acquisition, but upregulated heme protein genes. Transcriptional activity of the Gliotoxin cluster was low in all experiments, while the Fumagillin cluster showed substantial activity both on glucose and under carbon starvation, and the Hexadehydro-astechrome cluster only on glucose. We concluded that glucose withdrawal substantially modified the physiology of A. fumigatus, including processes that contribute to virulence. This may explain the challenge of predicting the in vivo behavior of A. fumigatus based on data from glucose rich cultures.

| S-EPMC9692504 | biostudies-literature

Effect of short-term glucose withdrawal and glucose refeeding after short-term glucose withdrawal on gene expression of MCF7 cells

Project description:Effect of short-term glucose withdrawal and glucose refeeding after short-term glucose withdrawal on gene expression of MCF7 cells

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Effect of short-term glucose withdrawal on gene expression of MCF7 cells

Project description:MCF7 is an epithelial cell line isolated from the breast tissue of a patient with metastatic adenocarcinoma. Whether glucose autoregulates its own metabolism remains poorly understood. To probe this, we performed RNA-Seq analysis on MCF7 breast cancer cells cultured with/without short-term glucose withdrawal.

2023-06-29 | GSE206393 | GEO

The oxidative stress response highly depends on glucose and iron availability in Aspergillus fumigatus

Project description:Pathogens have to cope with oxidative, iron- and carbon-limitation stresses in the human body. To understand how combined iron-carbon limitation alters oxidative stress response, Aspergillus fumigatus was cultured in glucose-peptone or peptone containing media supplemented or not with deferiprone as iron chelator. Changes in the transcriptome in these cultures were recorded after H2O2 treatment. Responses to oxidative stress were highly dependent on the availability of glucose and iron. Out of the 16 stress responsive antioxidative enzyme genes only the cat2 catalase-peroxidase gene was upregulated in more than two culturing conditions. The transcriptional responses observed in iron metabolism also varied substantially in these cultures. Only extracellular siderophore production appeared important regardless to culturing conditions in oxidative stress protection, while enhanced synthesis of Fe-S cluster proteins seemed to be crucial for oxidative stress treated iron-limited and fast growing (glucose rich) cultures. Although pathogens and host cells live together in the same place, their culturing conditions (e.g. iron availability, or occurrence of oxidative stress) can be different. Therefore, inhibition of a universally important biochemical process, like Fe-S cluster assembly, may selectively inhibit the pathogen growth in vivo and represent a potential target for antifungal therapy.

2024-04-03 | GSE256524 | GEO

Effect of glucose refeeding after short-term glucose withdrawal on gene expression of MCF7 cells

Project description:Effect of glucose refeeding after short-term glucose withdrawal on gene expression of MCF7 cells

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Effect of short-term glucose withdrawal on gene expression of MCF7 cells

Project description:Effect of short-term glucose withdrawal on gene expression of MCF7 cells

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The oxidative stress response highly depends on glucose and iron availability in Aspergillus fumigatus

Project description:The oxidative stress response highly depends on glucose and iron availability in Aspergillus fumigatus

| PRJNA1079828 | ENA

Gene expression level of Aspergillus fumigatus CEA17_ΔakuBKU80 at three different stages

Project description:We report the gene expression level of Aspergillus fumigatus CEA17_ΔakuBKU80 strain in dormant conidia, in swollen conidia (after 4h of culture in Glucose 3%, Yeast Exrtract 1% liquid medium) and in germinated conidia (after 8h of culture in Glucose 3%, Yeast Extract 1% liquid medium after construction of an RNAseq library.

2016-09-26 | GSE81956 | GEO

Comparative genomic hybridization of Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae and Neosartorya fischeri with Aspergillus fumigatus Af293

Project description:Genomic DNA from five strains, Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae, and Neosartorya fischeri, were co-hybridized with that of Aspergillus fumigatus Af293 and compared.

2005-05-15 | E-MEXP-333 | biostudies-arrayexpress

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