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Memory phase-specific genes identified by CrebB-target DamID in the Mushroom Bodies

ABSTRACT: Memory phase-specific genes identified by CrebB-target DamID in the Mushroom Bodies

PROVIDER: PRJNA891757 | ENA |

REPOSITORIES: ENA

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Memory phase-specific genes identified by CrebB-target DamID in the Mushroom Bodies

Project description:The formation of long-term memories requires changes in the transcriptional program and de novo protein synthesis. One of the critical regulators for long-term memory (LTM) formation and maintenance is the transcription factor CREB. Genetic studies have dissected the requirement of CREB activity within memory circuits, however less is known about the genetic mechanisms acting downstream of CREB and how they may contribute defining LTM phases. To better understand the downstream mechanisms, we here used a targeted DamID approach (TaDa). We generated a CREB-Dam fusion protein using the fruit fly Drosophila melanogaster as model. Expressing CREB-Dam in the mushroom bodies (MBs), a brain center implicated in olfactory memory formation, we identified genes that are differentially expressed between paired and unpaired appetitive training paradigm. Of those genes we selected candidates for an RNAi screen in which we identified genes causing increased or decreased LTM.

2022-10-19 | GSE216045 | GEO

Memory phase-specific genes in the Mushroom Bodies identified using CrebB-target DamID.

| S-EPMC10289670 | biostudies-literature

Expression profiling of Drosophila mushroom bodies

Project description:We performed mRNA-seq of dissected Drosophila mushroom bodies, comparing to whole brain and testis

2012-02-01 | GSE33783 | GEO

Expression profiling of Drosophila mushroom bodies

Project description:We performed mRNA-seq of dissected Drosophila mushroom bodies, comparing to whole brain and testis mRNA seq of MB, brain and testis

2012-02-01 | E-GEOD-33783 | biostudies-arrayexpress

Expression data from the mushroom bodies of Drosophila-PKA activity dependent manner

Project description:Temperature preference behavior in Drosophila depends on the level of PKA signaling in the mushroom bodies. To identify new components downstream to PKA, we carried out a genome-wide screen for genes regulated by PKA signaling in the mushroom bodies. Using the Gal4-UAS system, we increased or decreased PKA activity in the mushroom bodies by expressing dominant-negative (UAS-PKADN) or constitutively active PKA (UAS-PKACA), respectively. Expression of PKA transgenes was targeted to the mushroom bodies using the mushroom body-specific MB247-Gal4 driver. PKA expression was induced for 12-16 hours in three-day-old adults by inactivating the temperature-sensitive Gal80 at the restrictive temperature. We then analyzed gene-expression profiles to identify the genes showing altered expression levels in response to the high or low PKA activity.

2014-03-25 | E-GEOD-28181 | biostudies-arrayexpress

Expression data from the mushroom bodies of Drosophila-PKA activity dependent manner

2014-03-25 | GSE28181 | GEO

Transcription profiling of Drosophila melanogaster ablated mushroom bodies vs unablated MB to investigate the repertoire of MB genes that control initiation and maintenance of neural differentiation during learning and memory

Project description:Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. To get insights of the repertoire of MB genes that control initiation and maintenance of neural differentiation as well as the repertoire of neural factors that may have functions in the synaptic plasticity of MB neurons during learning and memory, we compared the transcript profiles between wild type and MB-ablated brains using a Drosophila whole-genome microarray. Newly hatched larvae were briefly administered with a DNA-synthesis inhibitor, hydroxyurea, and raised to adults, from which total brain RNA was analyzed. Experiment Overall Design: Two conditions analyzed: Control Brains and Musroom Body-ablated brains. Experiment Overall Design: Each condition was analyzed in triplicate.

2007-11-01 | E-GEOD-3379 | biostudies-arrayexpress

A Genome-Wide Survey of Drosophila Mushroom Body Transcripts with Chemical Ablation

Project description:Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. To get insights of the repertoire of MB genes that control initiation and maintenance of neural differentiation as well as the repertoire of neural factors that may have functions in the synaptic plasticity of MB neurons during learning and memory, we compared the transcript profiles between wild type and MB-ablated brains using a Drosophila whole-genome microarray. Newly hatched larvae were briefly administered with a DNA-synthesis inhibitor, hydroxyurea, and raised to adults, from which total brain RNA was analyzed. Keywords: Chemical Ablation of Mushroom bodies from Drosophila brain

2006-07-01 | GSE3379 | GEO

Mushroom body cell type comparison

Project description:The Drosophila melanogaster mushroom bodies (MBs) are brain structures critical for olfactory memory. The approximately 2000 intrinsic MB neurons are divisible into alpha/beta neurons, alpha'/beta' neurons and gamma neurons by morphology and roles in memory processing. It has also been shown that the different subtypes of MB neurons have different function in the process of memory formation. This difference in function, suggested to us that different types of MB neurons might have different expression profiles. In this study, we used cell-type specific gene expression profiling to gain insight into cellular properties of MB neurons.

2013-01-10 | E-MEXP-3798 | biostudies-arrayexpress

Nuclear transcriptomes of the seven neuronal cell types that constitute the Drosophila mushroom bodies

Project description:Nuclear transcriptomes of the seven neuronal cell types that constitute the Drosophila mushroom bodies

| PRJNA489789 | ENA