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Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract

ABSTRACT: Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract

PROVIDER: PRJNA892062 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21972035.fastq.gz	Fastqsanger.gz
	SRR21972036.fastq.gz	Fastqsanger.gz
	SRR21972037.fastq.gz	Fastqsanger.gz
	SRR21972038.fastq.gz	Fastqsanger.gz
	SRR21972039.fastq.gz	Fastqsanger.gz

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Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract [miRNA-seq]

Project description:The female’s reproductive tract is exposed directly to the male’s ejaculate, making it a hotspot for mating-induced responses shortly after mating. In Drosophila melanogaster, changes in the reproductive tract are essential to optimize fertilization. To detect the earliest gene regulatory events that underlie these changes, we measured transcript abundances using RNA-seq and microRNA-seq of reproductive tracts of unmated females and females collected within 10-15 minutes after the end of mating, either to a wildtype male or to a male with defective BMP signaling in secondary cells of the accessory gland, which influences the composition of the male’s ejaculate. We observed transcript abundance changes for genes with roles in tissue morphogenesis, wound healing, the immune response and metabolism. Strikingly, predicted targets of microRNAs that respond to mating are enriched for overlapping functions, suggesting that mating-induced changes are in part regulated by microRNAs. Most of the differentially expressed RNAs are upregulated in response to mating, while most of the differentially expressed microRNAs are downregulated. This pattern suggests a response of activation and de-repression of gene programs that switch the reproductive tract to a “mated” state, rather than a repression of virgin-specific programs. Male genotype did not influence transcript levels, indicating that the earliest transcriptomic responses in the reproductive tract are not dependent on ejaculate components that require BMP signaling in secondary cells. Our results shed light on the molecular changes that accompany very early responses to mating and present candidate genes and microRNAs that can be further examined for their participation in alterations of the reproductive tract microenvironment in response to signals from the male.

2023-06-27 | GSE216084 | GEO

Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract [mRNA-seq]

2023-06-27 | GSE216083 | GEO

Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract [mRNA-seq]

Project description:Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract [mRNA-seq]

| PRJNA892068 | ENA

Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract [miRNA-seq]

Project description:Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract [miRNA-seq]

| PRJNA892067 | ENA

Comparison of post-mating responses in the female reproductive tract among Drosophila species

Project description:Fertility depends on the coordination of complex interactions between male and female reproductive proteins inside the female reproductive tract (FRT). These interactions mediate a suite of changes in female behavior, morphology, and physiology after mating, yet little is known about how the molecular environment of the FRT may differ among species and coordinate species-specific female post-mating responses. We used semi-quantitative proteomics to compare the FRT protein composition between virgin and mated females in Drosophila melanogaster. These results are compared to those from quantitative TMT proteomic analyses of the mating-induced changes in D. simulans and D. mauritiana, and after conspecific and heterospecific inseminations. Our study highlights the value of using quantitative proteomics approaches to study the molecular composition of the FRT environment, and how its divergence may inform mechanistic studies of post-mating pre-zygotic reproductive isolation between species.

2020-06-25 | PXD017730 | Pride

Mating activates transcriptional programs and represses microRNAs in the Drosophila melanogaster female reproductive tract

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-06-27 | GSE216085 | GEO

Mating induced transcriptional changes in the female lower reproductive tract (LRT) of Anopheles gambiae mosquitoes at three time points (3, 12 and 24 hours) post mating.

Project description:To gain better insight into the molecular link between the post-mating transcriptional and morphological changes and the behavioral and physiological shift induced by copulation in An. gambiae females, we performed an in-depth transcriptional analysis of the female lower reproductive tract (LRT), comprising the atrium and the spermatheca, to identify the molecular pathways specifically regulated in these female reproductive tissues. We analyzed three time points post mating (3, 12 and 24 hours post mating) in order to capture transcriptional changes occurring within a broad time window after copulation. For each time point, tissues were collected from mated and age-matched virgin females and the transcriptional profile compared across 4 biological replicates using Agilent one-color microarrays.

2015-02-03 | E-MTAB-3022 | biostudies-arrayexpress

Drosophila female reproductive glands contribute to mating plug composition and the timing of sperm ejection

Project description:Reproductive traits that influence female remating and competitive fertilization rapidly evolve in response to sexual selection and sexual conflict. One such trait, observed across diverse animal taxa, is the formation of a structural plug inside the female reproductive tract, either during or shortly after mating. In Drosophila melanogaster, male seminal fluid forms a mating plug inside the female bursa, which has been demonstrated to influence sperm entry into storage and latency of female remating. Processing of the plug, including its eventual ejection from the female's reproductive tract, influences the competitive fertilization success of her mates and is mediated by female × male genotypic interactions. However, female contributions to plug formation and processing have received limited attention. Using developmental mutants that lack glandular female reproductive tract tissues, we reveal that these glandular tissues are essential for the mating plug to be ejected. We further use proteomics to demonstrate that female glandular proteins, and especially proteolytic enzymes, contribute to mating plug composition and that the absence of glands has a widespread impact of plug formation and composition. Together, these phenotypic and molecular data resolve molecular mechanisms of important postmating, intersexual interactions and cryptic female choice.

2022-01-25 | PXD028524 | Pride

Spatiotemporal female reproductive tract gene expression in Drosophila melanogaster

Project description:In order to better understand the female reproductive tract (FRT) we conducted a systematic, comprehensive investigation of the FRT in a tssue-specific manner at three time points relative to mating. By characterizing the transcriptional relationships among discrete FRT tissues across time we advance the understanding of the molecular genetics of FRT functions.

2020-12-29 | GSE143759 | GEO

mating response genes

Project description:Male-derived accessory gland proteins (Acps) that are transferred to females during mating have profound effects on female reproductive physiology including increased ovulation, mating inhibition, and effects on sperm utilization and storage. The extreme rates of evolution seen in Acps may be driven by sperm competition and sexual conflict, processes which may ultimately drive complex interactions between female- and male-derived molecules and sperm. However, little is known of how gene expression in female reproductive tissues changes in response to the presence of male molecules and sperm. To characterize this response, we conducted parallel genomic and proteomic analyses of gene expression in the reproductive tract of 3-day-old unmated and mated female Drosophila melanogaster. Using DNA microarrays, we identified 539 transcripts that are differentially expressed in unmated vs. mated females and revealed a striking peak in differential expression at 6 hrs postmating and a marked shift from primarily down-regulated to primarily up-regulated transcripts within 3 hrs after mating. Combining two-dimensional gel electrophoresis and liquid chromatography mass spectrometry analyses, we identified 84 differentially expressed proteins at 3 hrs postmating, including proteins which appeared to undergo post-translational modification. Together, our observations define transcriptional and translational response to mating within the female reproductive tract and suggest a bimodal model of postmating gene expression initially correlated with mating and the final stages of female reproductive tract maturation and later with the declining presence of male reproductive molecules and with sperm maintenance and utilization. Keywords: keywords: reproduction, reproductive tract, accessory gland proteins, sperm, timecourse

2006-11-03 | GSE5012 | GEO

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