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Assessment of the impact of the TLR3 L412F variant in terms of TLR3-sensitive transcript induction

ABSTRACT: Assessment of the impact of the TLR3 L412F variant in terms of TLR3-sensitive transcript induction

PROVIDER: PRJNA892959 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22007161_1.fastq.gz	Fastqsanger.gz
	SRR22007161_2.fastq.gz	Fastqsanger.gz
	SRR22007162_1.fastq.gz	Fastqsanger.gz
	SRR22007162_2.fastq.gz	Fastqsanger.gz
	SRR22007163_1.fastq.gz	Fastqsanger.gz

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Assessment of the impact of the TLR3 L412F variant in terms of TLR3-sensitive transcript induction

Project description:we assessed the impact of TLR3 L412F at endogenous cellular levels upon double stranded RNA (dsRNA) stimulation, by performing RNA-sequencing in primary fibroblasts from individuals homozygous for the ancestral allele (n =2) or homozygous for TLR3 L412F (n = 4) upon extracellular Poly(I:C) stimulation that activates the TLR3 pathway. The average response of TLR3 L412F homozygotes was significantly decreased (mean Δ log2 fold-change = 0.93; Wilcoxon p <10-16) compared to that of cells with the ancestral allele, approaching that of TLR3-deficient cells, used as negative control.

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