Project description:The objective of this study is to identify human meibomian gland genes that may promote the development and/or progression of meibomian gland dysfunction. Total RNA was isolated from normals (n=6) and patients with meibomian disease (n=6). The RNA was then used in conjuction with HumanHT-12 v3 Expression BeadChips to assay differential gene expression between normal and diseased meibomian glands.
Project description:The objective of this study is to identify human meibomian gland genes that may promote the development and/or progression of meibomian gland dysfunction.
Project description:Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in immortalized human meibomian gland epithelial cells. Analysis of regulation of immortalized human meibomian gland epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like meibomian gland cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation of numerous genes in response to dihydrotestosterone incubation of immortalized human meibomian gland epithelial cells. Total RNA was obtained from immortalized human meibomian gland epithelial cells treated for 72 hours with 10 nM dihydrotestosterone (n=3) or vehicle (n=3). The RNA was then used with Illumina HumanHT-12 v3 Expression BeadChips to determine the effect of DHT on gene expression in an immortalized human meibomian epithelial cell line developed in our laboratory.
Project description:Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in immortalized human meibomian gland epithelial cells. Analysis of regulation of immortalized human meibomian gland epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like meibomian gland cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation of numerous genes in response to dihydrotestosterone incubation of immortalized human meibomian gland epithelial cells.
2011-03-31 | GSE18091 | GEO
Project description:Analysis of conjunctival sac microbiome in meibomian gland dysfunction and mixed dry eye patients
Project description:Hyperlipidemia can induce the dysfunction of meibomian gland (MG) in mice, which may be affected by circadian rhythm. However, the underlying mechanism remains unclear. In this study, we exposed the hyperlipidemic mice model induced by three months feeding of high-fat diet to the regular light-dark cycles for two weeks. Then, phenotypic observation and RNA-seq of MG in experimental mice were performed to investigate the effect and transcriptional changes of hyperlipidemia and circadian rhythm on MG dysfunction. As a result, several significantly expressed genes and enriched pathways were identified to be associated with MG dysfunction in hyperlipidemic mice under circadian rhythms. High fat diet can not only bring hyperlipidemia, but also cause meibomian gland dysfunction, which is affected by rhythm genes. These data can provide us with a deeper understanding of the outcomes of MG altered by daily nutritional challenge.
Project description:Analysis of growth factor influence on immortalized human meibomian gland epithelial cells at gene expression level. Growth factors play a critical role in the proliferation and differentiation of sebaceous gland epithelial cells. Given that the meibomian gland is a large sebaceous gland, we hypothesize that growth factors exert analogous effects on human meibomian gland epithelial cells. Results provide important information of the response of human meibomian gland epithelial cells to epidermal growth factor (EGF), bovine pituitary extract (BPE), and both, such as up- or down- regulated genes involved in lipid metabolic process and cell cycle.
