Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Saccharomyces eubayanus

ABSTRACT: Genomic and transcriptomic analysis of Saccharomyces eubayanus isolates

PROVIDER: PRJNA894214 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22064351_1.fastq.gz	Fastqsanger.gz
	SRR22064351_2.fastq.gz	Fastqsanger.gz
	SRR22064352_1.fastq.gz	Fastqsanger.gz
	SRR22064352_2.fastq.gz	Fastqsanger.gz
	SRR22064353_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 71

Similar Datasets

Structural, physiological and regulatory analysis of maltose transporter genes in Saccharomyces eubayanus CBS12357T.

Project description:Saccharomyces pastorianus lager brewing yeasts are domesticated hybrids of Saccharomyces cerevisiae and cold-tolerant Saccharomyces eubayanus. To better understand the contribution of both parental genomes to maltose metabolism in brewing wort, this study focuses on maltose transport in the S. eubayanus type strain CBS12357T/FM1318T. To obtain complete sequences of the MAL loci of this strain, a near-complete genome assembly was generated using the Oxford Nanopore Technology MinION sequencing platform. Except for CHRXII, all sixteen chromosomes were assembled as single contigs. Four loci harboring putative maltose transporter genes (SeMALT1-4), located in subtelomeric regions of CHRII, CHRV, CHRXIII and CHRXVI, were completely resolved. The near-identical loci on CHRV and CHRXVI strongly resembled canonical S. cerevisiae MAL loci, while those on CHRII and CHRXIII showed different structures suggestive of gene loss. Functionality of the SeMALT1-4-encoded transporters was confirmed by their ability to restore growth on maltose, but not on maltotriose, of a maltose-transport-deficient S. cerevisiae strain. Simultaneous CRISPR-Cas9-assisted deletion of SeMALT2 and SeMALT4, which shared 99.7 % sequence identity, eliminated growth of S. eubayanus CBS12357T on maltose. Transcriptome analysis of S. eubayanus CBS12357T established that, in maltose-grown cultures, SeMALT2 and SeMALT4 were expressed at much higher levels than SeMALT1 and SeMALT3, thus resolving the apparent discrepancy between heterologous expression and deletion studies. These results represent a first genomic and physiological characterization of maltose transport in S. eubayanus CBS12357T and provides a valuable resource for further industrial exploitation of this yeast.

2018-07-18 | GSE117246 | GEO

Saccharomyces eubayanus

Project description:Saccharomyces eubayanus Raw sequence reads

| PRJNA290017 | ENA

Saccharomyces eubayanus

Project description:Saccharomyces eubayanus Raw sequence reads

| PRJNA555221 | ENA

Saccharomyces cerevisiae x Saccharomyces eubayanus

Project description:Saccharomyces cerevisiae x Saccharomyces eubayanus overview

| PRJNA492591 | ENA

Saccharomyces cerevisiae x Saccharomyces eubayanus

Project description:Saccharomyces cerevisiae x Saccharomyces eubayanus Raw sequence reads

| PRJNA381789 | ENA

Saccharomyces cerevisiae x Saccharomyces eubayanus

Project description:Saccharomyces cerevisiae x Saccharomyces eubayanus Raw sequence reads

| PRJNA301546 | ENA

Saccharomyces eubayanus strain:yHRVM108

Project description:Saccharomyces eubayanus yHRVM108 Resequencing genome sequencing

| PRJNA619068 | ENA

Saccharomyces eubayanus strain:yHEB1585

Project description:Saccharomyces eubayanus yHEB1585 Resequencing genome sequencing

| PRJNA619069 | ENA

Saccharomyces eubayanus strain:CBS12357

Project description:Saccharomyces eubayanus strain:CBS12357 Genome sequencing

| PRJNA264003 | ENA

Saccharomyces eubayanus strain:yHEB1588

Project description:Saccharomyces eubayanus yHEB1588 Resequencing genome sequencing

| PRJNA619066 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data