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Mycolicibacterium fortuitum strain:ATCC 35855 | isolate:ATCC 6842

ABSTRACT: Mycolicibacterium fortuitum strain:ATCC 35855 | isolate:ATCC 6842 Genome sequencing and assembly

PROVIDER: PRJNA894292 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR24187261_1.fastq.gz	Fastqsanger.gz
	SRR24187261_2.fastq.gz	Fastqsanger.gz
	SRR24187262_1.fastq.gz	Fastqsanger.gz
	SRR24187262_2.fastq.gz	Fastqsanger.gz

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Project description:Detection of species-specific proteotypic peptides for accurate and easy characterization of infectious non-tuberculous mycobacteria such as Mycobacterium fortuitum is essential. Therefore, we carried out an in-depth global proteomic experiment using M. fortuitum ATCC 6841 strain followed by a proteome database search and spectral library generation. The lysate was subjected to in-solution proteomic sample preparation and fractionated using an offline C18 StageTip. Each fraction was acquired in technical triplicates using a 180 min data-dependent acquisition (DDA) method in Orbitrap Fusion Tribrid (Thermo Scientific) mass spectrometer. The resulting raw DDA data were searched against the M. fortuitum proteome database using Proteome Discoverer and FragPipe. The resulting peptide spectrum matches were converted into a spectral library using BiblioSpec.

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