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Decoding the endometrial niche of Asherman's syndrome at single-cell resolution [organoids]

ABSTRACT: Decoding the endometrial niche of Asherman's syndrome at single-cell resolution [organoids]

PROVIDER: PRJNA895089 | ENA |

REPOSITORIES: ENA

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Decoding the endometrial niche of Asherman's syndrome at single-cell resolution [organoids]

Project description:Asherman’s Syndrome is characterized by intrauterine adhesions or scarring, which cause infertility, menstrual abnormalities, and recurrent pregnancy loss. The pathophysiology of this syndrome remains unknown, with treatment restricted to recurrent surgical removal of intrauterine scarring, which has limited success. Here, we decode the Asherman’s Syndrome endometrial cell niche by analyzing data from over 200,000 cells with single-cell RNA-sequencing in patients with this condition and through in vitro analyses of Asherman’s Syndrome patient-derived endometrial organoids. Our endometrial atlas highlights the loss of the endometrial epithelium, alterations to epithelial differentiation signaling pathways such as Wnt and Notch, and the appearance of characteristic epithelium expressing secretory leukocyte protease inhibitor during the window of implantation. We describe syndrome-associated alterations in cell-to-cell communication and gene expression profiles that support a dysfunctional pro-fibrotic, pro-inflammatory, and anti-angiogenic environment.

2023-08-03 | GSE216748 | GEO

Decoding the endometrial niche of Asherman's syndrome at single-cell resolution

Project description:Decoding the endometrial niche of Asherman's syndrome at single-cell resolution

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Decoding the endometrial niche of Asherman's syndrome at single-cell resolution

2023-08-03 | GSE215968 | GEO

Human endometrial organoids

Project description:Human endometrial organoids

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Human endometrial organoids

Project description:Human endometrial organoid cultures were established from the decidual samples. We analyzed the global expression profiles of the endometrial organoids. Next, we determined the global expression patterns upon estrogen treatment and compared them to untreated controls.

2021-01-27 | GSE128605 | GEO

Derivation of endometrial gland organoids from term placenta

Project description:A limitation of current methods for the generation of endometrial gland organoids is their reliance on decidua isolated from endometrial biopsies or elective abortion. Here we report the establishment of endometrial gland organoids from decidua isolated from term placental membranes. These organoids express typical markers of glandular epithelia such as E-cadherin, Laminin and Cytokeratin 7, and can be propagated in cell culture through multiple passages. Additionally, we identified potential survival factors for the co-culture of organoids and endometrial stromal fibroblasts. These modifications facilitate the generation of patient-specific endometrial gland organoids with known pregnancy outcomes.

2020-09-03 | GSE157380 | GEO

Transcriptomic profiling of patient-derived endometrial cancer organoids (Berg HF)

Project description:Patient-derived endometrial cancer organoids. The data was used to compare gene expression profile between organoids, and to explore whether an organoid-derived gene signature could predict disease outcomes in independent patient cohorts.

2021-07-16 | E-MTAB-10664 | biostudies-arrayexpress

Self-renewing Endometrial Epithelial Organoids of the Human Uterus [bulk RNA-seq data]

Project description:The inner lining of the human uterus, termed the endometrium, is replete with glands that are hypothesized to be important for establishment of pregnancy through interactions with other endometrial cells and the embryo. A three-dimensional culture system was utilized to establish and generate endometrial epithelial organoids from normal human endometrium that display long-term expandability, can be cryopreserved, and are hormone responsive. Here, single cell RNA-seq was used to create a high-resolution gene expression atlas of the endometrial epithelial organoids and to define responsiveness to reproductive hormones.

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Self-renewing Endometrial Epithelial Organoids of the Human Uterus [single-cell RNA-seq data]

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