Project description:Deep sequencing of total RNA extracted from the genital discs of males for each of the following strains : Drosophila sechellia, Drosophila mauritiana, hybrid introgression line 3Q1(A) and hybrid introgression line Q1(A)
Project description:Leaves and panicles from recurrent parent KMR3 and a high yielding KMR3-O.rufipogon introgression line were used Microarray analysis was carried out to reveal up- and down-regulated DEGs in leaves and panicles of control and experimental line for identifying key genes that help improve yield
Project description:Deep sequencing of total RNA extracted from the genital discs of males for each of the following strains : Drosophila sechellia, Drosophila mauritiana, hybrid introgression line 3Q1(A) and hybrid introgression line Q1(A) Analysis of poly(A)+ RNA for three independent biological replicates of sequencing libraries for each of the following strains: D. sechellia w, D. mauritiana P-insertion Q1, hybrid introgression line 3Q1(A), and hybrid introgression line Q1(A). Male genital discs were obtained as described above, and total RNA was extracted using RNAqueousM-CM-^BM-BM-.-Micro Kit (Ambion). Poly(A)+ transcripts were isolated subsequently using MicroPoly(A)PuristM-CM-"M-BM-^DM-BM-" Kit (Ambion). To facilitate normalization of reads across our samples, at this stage of library construction we spiked-in small amounts of exogenous RNA from ArrayControlM-CM-"M-BM-^DM-BM-" Kit (Ambion) into each sample of poly(A)+ RNA. Paired-end sequencing was carried out by loading the samples onto four lanes (three samples per lane) of a flow cell and run on an Illumina Genome Analyzer IIx sequencer using 72 cycles per end of each paired-end read. Biological replicates of each genotype were loaded onto separate lanes.
Project description:RNA was extracted from adult male and adult female Drosophila simulans carrying small genomic segments introgressed from Drosophila mauritiana Seven introgression genotypes were profiled, as well as the parental D. simulans and D. mauritiana strains. Each sex-by-genotype was assayed on four replicate arrays incorporating a dye swap
Project description:These arrays measure gene expression across eight Y introgression lines in Drosophila simulans. Four lines (Ya19, Ya23, Ya24, Ya26) carry a D. simulans Y chromosome (from a Cameroon population) and four lines (Sec01, Sec03, Sec08, Sec27) carry a D. sechellia Y chromosome. All lines are otherwise identical with a D. simulans background (UCSD stock center line 14021-0251.092).
2011-09-30 | GSE31907 | GEO
Project description:WGS of mitonuclear introgression lines of Drosophila melanogaster
| PRJNA853138 | ENA
Project description:Introgression of pigmentation genes between two Drosophila species
