Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Novel mode of half site binding in budding yeast I

ABSTRACT: A Novel mode of half site binding in budding yeast I

PROVIDER: PRJNA895474 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22085846_1.fastq.gz	Fastqsanger.gz
	SRR22085846_2.fastq.gz	Fastqsanger.gz
	SRR22085847_1.fastq.gz	Fastqsanger.gz
	SRR22085847_2.fastq.gz	Fastqsanger.gz
	SRR22085848_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 62

Similar Datasets

A Novel mode of half site binding in budding yeast

Project description:A Novel mode of half site binding in budding yeast

| PRJNA895465 | ENA

A Novel mode of half site binding in budding yeast II

Project description:A Novel mode of half site binding in budding yeast II

| PRJNA895477 | ENA

A Novel mode of half site binding in budding yeast II

Project description:Despite decades of research, predicting where a transcription factor (TF) binds using its sequences alone continues to be an outstanding problem. Here, our thermodynamic model of Gal4p-DNA binding revealed a new mechanism of binding site selection in which Gal4p can bind to promoters that lacks it consensus site. Further investigation revealed that these sequences that Gal4p unexpectedly binds to have a high density of its half site, CGG. Analyses showed the ability of CGG half sites to predict zinc cluster TF in vivo binding and exposed a clear relationship between CGG half sites and zinc cluster binding in multiple binding datasets. To dissect the molecular logic of this half site mode of binding we designed a Sort-seq library containing 6,798 yeast promoter sequences and measured their expression. We learned that there is a linear relationship between CGG half site density and zinc cluster driven expression in vivo. Additionally, we found that promoters encoding 10 half sites have the same transcriptional output as a single consensus site and found no clear effects of half site orientation and spacing on strength of activation. Our experiments demonstrate that this novel mode of half site binding is widespread across the yeast genome and expands our knowledge about TF binding specificity that can be applied to higher eukaryotes.

2022-11-04 | GSE216839 | GEO

A Novel mode of half site binding in budding yeast I

2022-11-04 | GSE216838 | GEO

A Novel mode of half site binding in budding yeast

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-11-04 | GSE216840 | GEO

Budding yeast mRNA export and processing factors

Project description:This SuperSeries is composed of the following subset Series: GSE30703: Budding yeast mRNA poly( A) site mapping GSE30705: Budding yeast mRNA export and processing factor localization Refer to individual Series

2011-09-24 | E-GEOD-30706 | biostudies-arrayexpress

RNAi in budding yeast

Project description:RNAi in budding yeast

| PRJNA119985 | ENA

DBP1 in budding yeast

Project description:DBP1 in budding yeast

| PRJNA436358 | ENA

Small RNAs in budding yeast

Project description:Small RNAs in budding yeast

| PRJNA123555 | ENA

Nucleosome exchange in budding yeast

Project description:Nucleosome exchange in budding yeast