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Comparison of gene profiles of human bone marrow stromal stem cell (hBMSCs) transfected by siRNA-Control and siRNA-KIAA1199

ABSTRACT: Comparison of gene profiles of human bone marrow stromal stem cell (hBMSCs) transfected by siRNA-Control and siRNA-KIAA1199

PROVIDER: PRJNA895829 | ENA |

REPOSITORIES: ENA

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Comparison of gene profiles of human bone marrow stromal stem cell (hBMSCs) transfected by siRNA-Control and siRNA-KIAA1199

Project description:human bone marrow stromal stem cells were transfected by siRNA-control (non-target) and siRNA-KIAA1199, after 72 hours, gene profiles were compared We used microarrays to detail the global programme of gene expression underlying cellularization and identified distinct classes of up-regulated genes during this process.

2022-10-31 | GSE216863 | GEO

Human Bone Marrow Stromal Cells cultured on polymer scaffolds with varied properties

2013-11-01 | GSE50743 | GEO

Human Bone Marrow Stromal Cells cultured on polymer scaffolds with varied properties

Project description:Microarray analysis was used to evaluate expression differences from a single donor human bone marrow stromal cells (hBMSCs) as a function of varied polymer-based tissue engineering scaffolds. The results revealed that gene expression patterns of hBMSCs grouped according to scaffold. A library of scaffolds prepared from polycaprolactone (PCL) or poly D,L-lactic acid (PDLLA) was sythesized and cultured with hBMSCs for 14 days with RNA extracted from cells on Day 1 and Day 14. Gene expression analysis was performed using BRB ArrayTools. GF = gas foam, SC = spun coat, BNF = big nanofiber, SNF = small nanofiber, FFF = free-form fabricated, TCPS = tissue culture polystyrene, TCPS+OS = tissue culture polystyrene with osteogenic supplements. The 72 arrays data was used previously in the publication: Kumar et al. The determination of stem cell fate by 3D scaffold structures through the control of cell shape, Biomaterials (2011) 32, 9188-9196. A companion data set of 24 arrays was submitted separately to GEO as GSE50744 and will be referenced to Baker et al. Ontology Analysis of Global Gene Expression Differences of Human Bone Marrow Stromal Cells Cultured on 3D Scaffolds or 2D Films

2013-11-01 | E-GEOD-50743 | biostudies-arrayexpress

human hTERT-RPE1 cell spheroid : Control siRNA transfected vs. YAP1 siRNA transfected

Project description:Transcriptional profiling of human hTERT-RPE1 cell spheroids comparing Control siRNA transfected hTERT-RPE1 cell spheroids with those transfected with YAP1 siRNA.

2015-02-01 | GSE54146 | GEO

Expression data from SHP specific siRNA or nonspecefic siRNA transfected rat astrocytes

Project description:To clarify the effect of SHP in LXRs-mediated signaling pathway, we performed global gene expression analysis of SHP siRNA transfected- or control siRNA transfected- astrocytes after IFN-γ and LXRs agonist. Microarray analysis revealed that expression of several genes encoding inflammatory mediators were reversed in SHP siRNA transfected-astrocytes, when compared with control siRNA transfected-astrocytes.

2016-01-21 | GSE76704 | GEO

Human Bone Marrow Stromal Cells cultured on 3D nanofiber scaffolds or 2D films

Project description:Microarray analysis was used to evaluate expression differences from a single donor human bone marrow stromal cells (hBMSCs) as a function of varied polymer-based tissue engineering scaffolds. These scaffolds include polycaprolactone (PCL) nanofibers (PCL_NF), films (PCL_SC), poly D,L-lactic acid (PDLLA) nanofibers (PDLLA_NF), films (PDLLA_SC), tissue culture polystyrene (TCPS) and TCPS with osteogenic supplements (TCPS_OS). The results revealed that scaffold structure was able to significantly affect gene expression, with nanofiber scaffolds inducing similar gene expression patterns to hBMCSs cultured with osteogenic media. A library of scaffolds prepared from polycaprolactone or poly D,L-lactic acid was sythesized and cultured with hBMSCs for 14 days with RNA extracted from cells on Day 1 and Day 14. Gene expression analysis was performed using BRB ArrayTools. SC = spun coat, BNF = big nanofiber, TCPS = tissue culture polystyrene, TCPS+OS = tissue culture polystyrene with osteogenic supplements. This data forms is part of a pending publication: Baker et al. Ontology Analysis of Global Gene Expression Differences of Human Bone Marrow Stromal Cells Cultured on 3D Scaffolds or 2D Films and is a subset of the 72 array data referenced in ( Kumar et al. The determination of stem cell fate by 3D scaffold structures through the control of cell shape, Biomaterials (2011) 32, 9188-9196.) The 72 array data set is submitted separately to GEO as GSE50743.

2013-11-01 | E-GEOD-50744 | biostudies-arrayexpress

Expression data from SHP specific siRNA or nonspecefic siRNA transfected rat astrocytes [extended study]

2016-06-04 | GSE82247 | GEO

SAS cells: control siRNA (siC) vs DDX3 siRNA (siD) transfected

Project description:SAS cells: control siRNA (siC) vs DDX3 siRNA (siD) transfected

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RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells

Project description:RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells

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RNA sequencing facilitates quantitative analysis of siRNA circHIPK2 transfected neural stem cells and siRNA circCon transfected neural stem cells transcriptomes

Project description:We report the application of Illumina paired-end RNA-seq approach for transcriptome of siRNA circHIPK2 transfected neural stem cells and siRNA circCon transfected neural stem cells . By removing sequence-dependent bias and amplification noise using UMI-tools. The mapped reads of each sample were assembled using StringTie. After the final transcriptome was generated, StringTie and edgeR was used to estimate the expression levels of all transcripts. By obtaining a total of million paired-end reads of sequence from neural stem cells , we generated transcriptome profiles of siRNA circHIPK2 transfected neural stem cells and siRNA circCon transfected neural stem cells , respectively. We found 201 differentially expressed genes (DEGs) between siRNA circHIPK2 transfected neural stem cells and siRNA circCon transfected neural stem cells. This study provides a detailed analysis of the underlying mechanisms of stroke，such as neuronal injury and long-term effect, generated by RNA-seq technology.

2020-02-24 | GSE133701 | GEO

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