Ontology highlight
ABSTRACT:
PROVIDER: PRJNA896770 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:DCMU, or diuron, is a widely used herbicide, which can cause adverse effects on human. Here, we develop an approach that combines functional, miRNA and RNA sequencing data to evaluate the effects of DCMU exposure on the human immune system, notably on CD8+ T cells, as the main effectors of the organism’s defense against viruses or neoplastic cells. We found that DCMU reduces T cell abilities, in vitro but also in vivo in orthotopic mouse and zebrafish models, participating to the establishment of a suitable environment for the development of more serious diseases such as cancers. We identified several miRNAs, such as hsa-miR3135b and hsa-miR-21-5p, respectively induced and repressed by DCMU, which alters CD8+ T cell functions. The major advancement of our study is the description of the molecular mechanisms induced by DCMU that control human immune functions.
2022-07-05 | GSE189443 | GEO
Project description:DCMU, or diuron, is a widely used herbicide, which can cause adverse effects on human. Here, we develop an approach that combines functional, miRNA and RNA sequencing data to evaluate the effects of DCMU exposure on the human immune system, notably on CD8+ T cells, as the main effectors of the organism’s defense against viruses or neoplastic cells. We found that DCMU reduces T cell abilities, in vitro but also in vivo in orthotopic mouse and zebrafish models, participating to the establishment of a suitable environment for the development of more serious diseases such as cancers. We identified several miRNAs, such as hsa-miR3135b and hsa-miR-21-5p, respectively induced and repressed by DCMU, which alters CD8+ T cell functions. The major advancement of our study is the description of the molecular mechanisms induced by DCMU that control human immune functions.
2022-07-05 | GSE189440 | GEO
Project description:Microalgae, particularly Chlamydomonas, produce valuable pigments like chlorophylls and carotenoids for industrial applications while demonstrating potential in bioremediation. This study employs proteomics to analyze protein expression patterns between norflurazon-resistant and non-resistant Chlamydomonas strains. The investigation aims to understand the molecular mechanisms underlying herbicide resistance, focusing on differential protein expression when exposed to norflurazon, a commonly used herbicide in weed control. This research could provide insights into both bioremediation capabilities and herbicide resistance mechanisms in microalgae.
2024-11-21 | PXD057458 | JPOST Repository
Project description:Here, we transferred GmDof4 from soybean (Glycine max), a transcription factor affecting content of lipid in Arabidopsis, into C. ellipsoidea and found that the expression of GmDof4 significantly enhanced the lipid content by maximum 57.21%, shifted the content of different fatty acids, but did not affect the growth of the host cells. Using the Solexa/Illumina-based RNA-Seq analysis, we found expression of GmDof4 significantly regulates 1,076 genes, of which 754 genes were up-regulated and 322 genes were down-regulated, under mixotrophic culture. This study provides a new way to improve the lipid of microalgae.
2014-04-16 | GSE37473 | GEO
Project description:Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use for biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the only energy source. Some diatoms, however, can also grow in mixotrophic mode, where both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum, using glycerol as a source of reduced carbon. Transcriptomic, metabolomic and physiological data indicate that glycerol affects the central-carbon, carbon-storage and lipid metabolism of the diatom. In particular, glycerol addition mimics some typical responses of nitrogen limitation on lipid metabolism at the level of TAG accumulation and fatty acid composition. However, this compound does not diminish photosynthetic activity and cell growth, at variance with nutrient limitation, revealing essential aspects of the metabolic flexibility of these microalgae and suggesting possible biotechnological applications of mixotrophy.
2017-03-31 | GSE91004 | GEO
Project description:Volatile fatty acids found in effluents of the dark fermentation of biowastes can be used for mixotrophic growth of microalgae, improving productivity and reducing the cost of the feedstock. Microalgae can use the acetate in the effluents very well, but butyrate is poorly assimilated and can inhibit growth above 1 gC.L-1. The non-photosynthetic chlorophyte alga Polytomella sp. SAG 198.80 was found to be able to assimilate butyrate fast. To decipher the metabolic pathways implicated in butyrate assimilation, a large-scale differential proteomics study was developed comparing Polytomella sp. cells grown on acetate and butyrate at 1 gC.L-1.
2022-11-02 | PXD035155 | Pride
Project description:Here, we transferred GmDof4 from soybean (Glycine max), a transcription factor affecting content of lipid in Arabidopsis, into C. ellipsoidea and found that the expression of GmDof4 significantly enhanced the lipid content by maximum 57.21%, shifted the content of different fatty acids, but did not affect the growth of the host cells. Using the Solexa/Illumina-based RNA-Seq analysis, we found expression of GmDof4 significantly regulates 1,076 genes, of which 754 genes were up-regulated and 322 genes were down-regulated, under mixotrophic culture. This study provides a new way to improve the lipid of microalgae. The GmDof4 transgenic strain Dof4-1 and control (pCK transgenic line CK-1) were cultured in liquid antibiotic-free Endo medium at 25M-bM-^DM-^C under illumination (100 M-NM-<mol photons m-2 s-1) for 120 h. Cells at a concentration of approximately 1M-CM-^W 10^7 cells/mL were collected for library construction and sequencing using Illumina GAIIx.
2014-04-16 | E-GEOD-37473 | biostudies-arrayexpress
Project description:Impact of dark fermentation effluent on microalgae growth in mixotrophic conditions
| PRJNA1107359 | ENA
Project description:Series of 6 repetitions of hybridization of treatment (PSII_DCMU) and control (PSI) each. Comparison of plants grown under PSII-specific light and treated with the electron transport inhibitor DCMU versus plants grown under PSI-specific light without DCMU treatment. T. Pfannschmidt, unpublished Keywords: repeat sample
2005-01-01 | GSE1168 | GEO