Ontology highlight
ABSTRACT:
PROVIDER: PRJNA897004 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Motor neurons (MNs) are the final output of circuits driving fundamental behaviors, such as respiration and locomotion. Hox proteins are essential in generating the MN diversity required for accomplishing these functions, but the transcriptional mechanisms that enable Hox paralogs to assign distinct MN subtype identities despite their promiscuous DNA binding motif are not well understood. Here we show that Hoxa5 can modify chromatin accessibility in all mouse spinal cervical MN subtypes and engages TALE co-factors to directly bind and regulate subtype-specific genes. We identify a paralog-specific interaction of Hoxa5 with the phrenic MN-specific transcription factor Scip and show that heterologous expression of Hoxa5 and Scip is sufficient to suppress limb-innervating MN identity. We also demonstrate that phrenic MN identity is stable after Hoxa5 downregulation and identify Klf proteins as potential regulators of phrenic MN maintenance. Our data identify multiple modes of Hoxa5 action that converge to induce and maintain MN identity.
2024-08-12 | GSE273363 | GEO
Project description:Motor neurons (MNs) are the final output of circuits driving fundamental behaviors, such as respiration and locomotion. Hox proteins are essential in generating the MN diversity required for accomplishing these functions, but the transcriptional mechanisms that enable Hox paralogs to assign distinct MN subtype identities despite their promiscuous DNA binding motif are not well understood. Here we show that Hoxa5 can modify chromatin accessibility in all mouse spinal cervical MN subtypes and engages TALE co-factors to directly bind and regulate subtype-specific genes. We identify a paralog-specific interaction of Hoxa5 with the phrenic MN-specific transcription factor Scip and show that heterologous expression of Hoxa5 and Scip is sufficient to suppress limb-innervating MN identity. We also demonstrate that phrenic MN identity is stable after Hoxa5 downregulation and identify Klf proteins as potential regulators of phrenic MN maintenance. Our data identify multiple modes of Hoxa5 action that converge to induce and maintain MN identity.
2024-08-12 | GSE273362 | GEO
Project description:Motor neurons (MNs) are the cellular targets of multiple adult-onset diseases. Because distinct MN populations differ in disease susceptibility, it is important to define in animal models the degree of diversity within adult MNs. Here, we generated a comprehensive molecular resource of adult MNs in C. elegans. Single-cell RNA-sequencing of 12,603 cells revealed that all eight morphologically defined MN classes of the ventral nerve cord and its flanking ganglia subdivide into 29 distinct subclasses, almost quadrupling the degree of their previously described diversity. We find that four of the six C. elegans Hox genes delineate most, but not all MN subclasses. Strikingly, all 29 subclasses are delineated by unique expression codes of neuropeptide genes and receptors, critical for extra-synaptic (wireless) signaling. Leveraging the C. elegans connectome, we found a strong correlation between molecularly and connectivity defined MN subclasses. Beyond providing a valuable resource and searchable database (spinalcordatlas.com), our study identifies Hox and neuropeptide codes as key molecular descriptors of adult MN diversity, codes likely conserved in MNs across species.
2023-06-26 | GSE234962 | GEO
Project description:microbial community diversity in nitrosamine-containing drinking water biofilters
| PRJNA264443 | ENA