Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiles in poh1-deficient murine pancreas

ABSTRACT: Gene expression profiles in poh1-deficient murine pancreas

PROVIDER: PRJNA897922 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22179229.fastq.gz	Fastqsanger.gz
	SRR22179229_1.fastq.gz	Fastqsanger.gz
	SRR22179229_2.fastq.gz	Fastqsanger.gz
	SRR22179230.fastq.gz	Fastqsanger.gz
	SRR22179230_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Gene expression profiles in poh1-deficient murine pancreas

Project description:To investigate the molecular events essentifal for POH1-mediated ADM and PDAC development, we performed RNA-seq assays to detect transcriptome changes in POH1-deficient pancreas.

2024-05-31 | GSE217219 | GEO

Basal gene expression in Daxx deficient murine pancreas

Project description:Basal gene expression in Daxx deficient murine pancreas

| PRJNA630898 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and poh1-/- splenic foxp3＋Treg cells

Project description:Purpose: The goals of this study are to compare the transcriptome profiling of Wild Type and poh1-/- splenic foxp3＋Treg cells Methods:Splenic CD4+Foxp3+ (YFP+) cells mRNA profiles of 2-week-old poh1+/+ Foxp3Cre+ wild type and poh1fl/fl Foxp3Cre+ mice were generated by deep sequencing, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed with edgeR. qRT–PCR validation was performed using SYBR Green assays Results:Using an optimized data analysis workflow, we mapped about 50 million sequence reads per sample to the mouse genome (build mm10). Among all genes expressed, 1067 genes were upregulated and1391 genes were downregulated in poh1−/− Foxp3+ Treg cells relative to their expression in POH1 +/+ Treg cells. The gene-expression altered in POH1-ablated cells adversely correlated with those expressed in Treg cells in TCR-dependent manner. The gene sets were downreglated in POH1-deficiency Treg cells encoding various cell-surface receptors and intracellular molecules involved in migration, suppressive function and signature of Treg cells. Conclusions:Gene expression in POH1-deficiency Treg cells was markedly diverse compared with that in POH1-sufficient Treg cells.

2020-12-12 | GSE110084 | GEO

Affymetrix Gene Expression profiles of murine LAMTOR2-deficient hematopoietic progenitor cells

Project description:Affymetrix Gene Expression profiles of murine LAMTOR2-deficient hematopoietic progenitor cells

| PRJNA412650 | ENA

Expression profiling analysis to identify POH1-regulated genes

Project description:This work determined the POH1 regulated genes in the absence or presence of TGF-β in SMMC-7721 cells through genome-wide transcriptome sequencing.

2020-12-12 | GSE109788 | GEO

The transcriptomes of PC3 cells with or without POH1 interference

Project description:Total RNA was obtained from PC3 cells with or without POH1 interference to perform a High-throughput sequencing

2021-09-25 | GSE120481 | GEO

POH1 regulates MYC in pancreatic cancer cells [CUT&TAG]

Project description:CUT&Tag experiments were used to analyze DNA-binding of MYC protein in the vector or POH1-Flag expressed mouse pancreatic cancer cells.

2024-11-01 | GSE217221 | GEO

Proteomic Analysis of Rap1A GTPase Signaling-Deficient C57BL/6 Mouse Pancreas and Functional Studies Identify an Essential Role of Rap1A in Pancreas Physiology

Project description:The Ras-related Rap1A GTPase is implicated in pancreas β-cell insulin secretion, and is stimulated by the cAMP sensor Epac2, a guanine exchange factor and activator of Rap1 GTPase. In this study we examined the differential proteomic profiles by nanoLC-ESI-MS/MS of pancreata from C57BL/6 Rap1A-deficient (Null) and control wild-type (WT) mice, to assess targets of Rap1A potentially involved in insulin regulation. We identified 77 overlapping identifier proteins in both groups, 8 distinct identifier proteins in Null versus 56 distinct identifier proteins in WT mice pancreas. Functional enrichment analysis showed 4 of the 8 Null unique proteins, ERO1-like protein β (Ero1lβ), triosephosphate isomerase (TP1), 14-3-3 protein γ and kallikrein-1, were exclusively involved in insulin biogenesis, with role in insulin metabolism. Specifically, the mRNA expression of Ero1lβ and TP1 was significantly (p<0.05) increased in Null versus WT pancreas. Rap1A-deficiency significantly affected glucose tolerance during the first 15-30 min of glucose challenge, but showed no impact on insulin sensitivity. Ex vivo glucose-stimulated insulin secretion (GSIS) studies on isolated Null islets showed significantly impaired GSIS. Furthermore, in GSIS-impaired islets, the cAMP-Epac2-Rap1A pathway was significantly compromised as compared to WT. Altogether, these studies underscore an essential role of Rap1A GTPase in pancreas physiological function

2024-10-17 | PXD052896 | Pride

Basal gene expression in Daxx deficient murine pancreas

Project description:We conducted basal gene expression analysis from total pancreas isolated from six week old mice

2020-05-08 | GSE150017 | GEO

RNAseq analysis of islets from murine Abca12 KO pancreas

Project description:RNAseq analysis of islets from murine Abca12 KO pancreas

| PRJNA589540 | ENA