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IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury (Tie2-Cre RiboTAG)

ABSTRACT: IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury (Tie2-Cre RiboTAG)

PROVIDER: PRJNA898432 | ENA |

REPOSITORIES: ENA

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IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury (Tie2-Cre RiboTAG)

Project description:Cerebral small vessel disease and brain white matter injury are worsened by cardiovascular risk factors including obesity. Molecular pathways in cerebral endothelial cells activated by chronic cerebrovascular risk factors alter cell-cell signaling, blocking endogenous and post-ischemic white matter repair. Using cell-specific translating ribosome affinity purification(RiboTag) in white matter endothelia and oligodendrocyte progenitor cells (OPCs), we identify a coordinated interleukin-chemokine signaling cascade within the oligovascular niche of subcortical white matter that is triggered by diet-induced obesity (DIO).DIO induces IL-17B signaling that acts on the cerebral endothelia through IL-17Rb to increase both circulating and local endothelial expression of CXCL5. In white matter endothelia, CXCL5 promotes the association of OPCs with the vasculature and triggers OPC gene expression programs regulating cell migration through chemokine signaling. Targeted blockade of IL-17B reduced vessel-associated OPCs by reducing endothelial CXCL5 expression. In multiple human cohorts, blood levels of CXCL5 function as a diagnostic and prognostic biomarker of vascular cognitive impairment.

2022-11-11 | GSE217355 | GEO

IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury (OPC RiboTAG)

2022-11-11 | GSE217354 | GEO

ROSA26Foxf1; Tie2-cre lung gene expression analysis.

Project description:Analysis of genes deregulated due to heterozygous overexpression of Foxf1 in endothelial and hematopoietic cells using Tie2-cre in embryonic day 18.5 mouse lungs. Total RNA obtained from three ROSA26Foxf1; Tie2-cre mouse lungs compared to three control ROSA26-lox-stop-lox (LSL) -Foxf1 mouse lungs.

2016-09-01 | E-GEOD-77881 | biostudies-arrayexpress

ROSA26Foxf1; Tie2-cre lung gene expression analysis.

Project description:Analysis of genes deregulated due to heterozygous overexpression of Foxf1 in endothelial and hematopoietic cells using Tie2-cre in embryonic day 18.5 mouse lungs.

2016-09-01 | GSE77881 | GEO

IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury (OPC RiboTAG)

Project description:IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury (OPC RiboTAG)

| PRJNA898430 | ENA

Tek+ cells from the E10 AGM region of Rictorf/f; Tie2-cre and control

Project description:we performed RNAseq analysis on Rictorf/f; Tie2-cre and control tek+ cells, to determine which hematopoietic genes are activated in the endothelium in the AGM by Rictor.

2017-07-30 | GSE59929 | GEO

RiboTag analysis of actively translated mRNAs in Sertoli and Leydig cells in vivo

Project description:Analysis of Sertoli and Leydig cell “translatome” utilizing an in vivo ribosome tagging strategy (RiboTag) that allows a detailed and physiologically relevant characterization of the polysome-associated mRNAs in vivo. Although progress has been made in the identification of specific transcripts that are translated in Sertoli and Leydig cells and their response to hormones, efforts to expand these studies have been restricted by technical hurdles. Our analysis identified all previously characterized Leydig and Sertoli cell-specific markers and identified in a comprehensive manner novel markers of Leydig and Sertoli cells; the translational response of these two cell types to gonadotropins or testosterone was also investigated. Leydig cell-specific (Cyp17iCre: RiboTag) and Sertoli cell-specific (AMH-Cre: RiboTag) RiboTag mice were obtained by crossing RiboTag homozygous mice with Cyp17iCre or AMH-Cre mice. For in vivo LH treatment experiments, mice were injected with the GnRH antagonist acyline for 4 days before a single injection of purified human LH. After treatment, testes were homogenized and polysomes were immunoprecipitated by utilizing an anti-HA antibody. RNA was extracted, labelled, and hybridized to Mouse Gene ST 1.0 arrays.

2013-04-06 | E-GEOD-45799 | biostudies-arrayexpress

RiboTag analysis of Gfi1Cre/Cre and Gfi1Cre/+ cochlear and vestibular hair cells

Project description:In order to identify the transcriptional cascade downstream of the GFI1 repressor in inner ear hair cells, we performed a RiboTag-based expression analysis of Gfi1cre/cre mutant hair cells compared to their heterozygous controls. Through this analysis, we identified a significant downregulation of genes involved in normal hair cell development and function within the mutant hair cells, suggesting that in the absence of GFI1, the hair cells undergo a maturation arrest. Furthermore, the Gfi1 deficient hair cells displayed a significant upregulation of genes involved in neurodifferentiation and neuronal function.

2020-08-01 | GSE135760 | GEO

RiboTag analysis of Gfi1Cre/Cre and Gfi1Cre/+ cochlear and vestibular hair cells

Project description:RiboTag analysis of Gfi1Cre/Cre and Gfi1Cre/+ cochlear and vestibular hair cells

| PRJNA559929 | ENA

Re-evaluating Microglia Expression Profiles Using RiboTag and Cell Isolation Strategies

Project description:Transcriptome profiling is widely used to infer functional states of specific cell types, as well as their responses to stimuli, to define contributions to physiology and pathophysiology. Focusing on microglia, the brain macrophages, we report here a side-by-side comparison of classical cell sort-based transcriptome sequencing and the ‘RiboTag’ method that avoids cell retrieval from tissue context and yields translatome sequencing information. Conventional whole cell microglia transcriptomes were found to be significantly tainted by artifacts induced by tissue-dissociation, cargo contaminations and transcripts sequestered from ribosomes. Conversely, our data highlight the added value of RiboTag profiling to assess the accuracy of Cre transgenic mice. Collectively, this study indicates method-based biases, reveals observer effects and establishes RiboTag-based translatome profiling as a valuable complement to standard sort-based profiling strategies.

2018-05-04 | GSE114001 | GEO

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