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Mt-Ty 5´tiRNA regulates skeletal muscle cell proliferation and differentiation

ABSTRACT: mt-Ty 5´tiRNA regulates skeletal muscle cell proliferation and differentiation

PROVIDER: PRJNA899504 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22230115_1.fastq.gz	Fastqsanger.gz
	SRR22230115_2.fastq.gz	Fastqsanger.gz
	SRR22230116_1.fastq.gz	Fastqsanger.gz
	SRR22230116_2.fastq.gz	Fastqsanger.gz
	SRR22230117_1.fastq.gz	Fastqsanger.gz

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mt-Ty 5´tiRNA regulates skeletal muscle cell proliferation and differentiation

Project description:Understanding the molecular mechanisms underlying the regulation of gene expression during skeletal muscle cell proliferation and differentiation is critical to the development of novel therapeutic targets to treat muscle disorders. In this study, we report the identification and characterization of mt-Ty 5’tiRNAs, tRNA-derived fragments (tiRNAs) we identified in skeletal muscle. mt-Ty 5’tiRNAs are found in the nucleus and mitochondria of skeletal muscle cells and its expression is induced by angiogenin. Gapmer-mediated inhibition of tiRNAs in skeletal muscle C2C12 myoblasts resulted in mitochondrial fragmentation. We also observed a decrease in cell proliferation and myogenic differentiation upon mt-Ty 5’tiRNA inhibition; consistent with this observation, RNA-seq and transcriptome analyses revealed that skeletal muscle cell differentiation and cell proliferation pathways were also downregulated. Conversely, overexpression of mt-Ty 5’tiRNAs in C2C12 cells led to a reversal of the transcriptional trends noted for these pathway genes. These data reveal mt-Ty 5’tiRNAs as a skeletal muscle enriched tiRNA that plays important roles in myoblast proliferation and differentiation. Our study also highlights the potential for the development of tiRNAs as novel therapeutic targets for muscle related diseases

2023-01-01 | GSE217539 | GEO

mt-Ty 5'tiRNA regulates skeletal muscle cell proliferation and differentiation.

Project description:In this study, we sought to determine the role of tRNA-derived fragments in the regulation of gene expression during skeletal muscle cell proliferation and differentiation. We employed cell culture to examine the function of mt-Ty 5' tiRNAs. Northern blotting, RT-PCR as well as RNA-Seq, were performed to determine the effects of mt-Ty 5' tiRNA loss and gain on gene expression. Standard and transmission electron microscopy (TEM) were used to characterize cell and sub-cellular structures. mt-Ty 5'tiRNAs were found to be enriched in mouse skeletal muscle, showing increased levels in later developmental stages. Gapmer-mediated inhibition of tiRNAs in skeletal muscle C2C12 myoblasts resulted in decreased cell proliferation and myogenic differentiation; consistent with this observation, RNA-Seq, transcriptome analyses, and RT-PCR revealed that skeletal muscle cell differentiation and cell proliferation pathways were also downregulated. Conversely, overexpression of mt-Ty 5'tiRNAs in C2C12 cells led to a reversal of these transcriptional trends. These data reveal that mt-Ty 5'tiRNAs are enriched in skeletal muscle and play an important role in myoblast proliferation and differentiation. Our study also highlights the potential for the development of tiRNAs as novel therapeutic targets for muscle-related diseases.

| S-EPMC10392060 | biostudies-literature

mt-Ty

Project description:Mus musculus mt-Ty, mitochondrially encoded tRNA tyrosine [Source:MGI Symbol;Acc:MGI:102470], is differentially expressed in 1 experiment(s);

| ENSMUSG00000064350 | ExpressionAtlas

mt-Ty

Project description:Mus musculus mt-Ty, mitochondrially encoded tRNA tyrosine [Source:MGI Symbol;Acc:MGI:102470], is expressed in 19 baseline experiment(s);

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MT-TY

Project description:Homo sapiens MT-TY, mitochondrially encoded tRNA-Tyr (UAU/C) [Source:HGNC Symbol;Acc:HGNC:7502], is differentially expressed in 32 experiment(s);

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MT-TY

Project description:Homo sapiens MT-TY, mitochondrially encoded tRNA-Tyr (UAU/C) [Source:HGNC Symbol;Acc:HGNC:7502], is expressed in 35 baseline experiment(s);

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Clostridium difficile CD630E JIR8094: growth 10h with 0.5% glucose in TY vs growth 10h in TY

Project description:transcriptionnal profiling of a C. difficile strain JIR8094 comparing growth 10h in 0.5% TY medium with growth 10h in TY medium

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Clostridium difficile mutant ccpA CD630E JIR8094: growth 10h with 0.5% glucose in TY vs growth 10h in TY

Project description:transcriptionnal profiling of a ccpA mutant of C. difficile strain JIR8094 comparing growth 10h in 0.5% TY medium with growth 10h in TY medium

2012-10-02 | GSE35073 | GEO

Sphingomonas melonis TY strain:TY

Project description:Sphingomonas melonis TY strain:TY Genome sequencing and assembly

| PRJNA307127 | ENA

Clostridium difficile CD630E JIR8094: growth 10h with 0.5% glucose in TY vs growth 10h in TY

Project description:transcriptionnal profiling of a C. difficile strain JIR8094 comparing growth 10h in 0.5% TY medium with growth 10h in TY medium two-conditions experiments, 0.5% glucose TY for 10h vs TY for 10h, 4 biological replicates for each condition, in an indirect design using a 8h TY RNA preparation as Reference

2012-10-02 | E-GEOD-35072 | biostudies-arrayexpress

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