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Chromatin states at homeoprotein loci distinguish axolotl limb segments prior to regeneration

ABSTRACT: Chromatin states at homeoprotein loci distinguish axolotl limb segments prior to regeneration

PROVIDER: PRJNA899785 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22244900_1.fastq.gz	Fastqsanger.gz
	SRR22244900_2.fastq.gz	Fastqsanger.gz
	SRR22245300_1.fastq.gz	Fastqsanger.gz
	SRR22245300_2.fastq.gz	Fastqsanger.gz
	SRR22244901_1.fastq.gz	Fastqsanger.gz

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Chromatin states at homeoprotein loci distinguish axolotl limb segments prior to regeneration [RNA-seq]

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Chromatin states at homeoprotein loci distinguish axolotl limb segments prior to regeneration [ATAC-seq]

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Project description:The salamander limb regenerates only the missing portion. Each limb segment can only form segments equivalent to- or more distal to their own identity, relying on a property termed “positional information”. How positional information is encoded in limb cells has been unknown. By cell-type-specific chromatin profiling of upper arm, lower arm, and hand, we found segment-specific levels of histone H3K27me3 at limb homeoprotein gene loci but not their upstream regulators, constituting an intrinsic segment information code. During regeneration, regeneration-specific regulatory elements became active prior to the re-appearance of developmental regulatory elements. This means that, in the hand segment, the permissive chromatin state of the hand homeoprotein gene HoxA13 engages with regeneration regulatory elements, bypassing the upper limb program. Profiling histone marks of Axoltol limb connective tissue cells, and comparing among limb different limb segments and mature vs regeneration time points.

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Project description:The salamander limb regenerates only the missing portion. Each limb segment can only form segments equivalent to- or more distal to their own identity, relying on a property termed “positional information”. How positional information is encoded in limb cells has been unknown. By cell-type-specific chromatin profiling of upper arm, lower arm, and hand, we found segment-specific levels of histone H3K27me3 at limb homeoprotein gene loci but not their upstream regulators, constituting an intrinsic segment information code. During regeneration, regeneration-specific regulatory elements became active prior to the re-appearance of developmental regulatory elements. This means that, in the hand segment, the permissive chromatin state of the hand homeoprotein gene HoxA13 engages with regeneration regulatory elements, bypassing the upper limb program. We performed Differential Gene expression analysis using data obtained from RNA-seq of two mature limb segments (mature arm vs hand). Therefore, we performed libraries from the mature arm and 5, 9, 13 dpa regenerating arm blastema.

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Chromatin states at homeoprotein loci distinguish axolotl limb segments prior to regeneration

Project description:This SuperSeries is composed of the SubSeries listed below.

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