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ABSTRACT: CD3+ Single cell in COVID-19 patients treated with nasal administration of anti-CD3 monoclonal antibody (Foralumab)

PROVIDER: PRJNA899867 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22247100.fastq.gz	Fastqsanger.gz
	SRR22247101.fastq.gz	Fastqsanger.gz
	SRR22247102.fastq.gz	Fastqsanger.gz
	SRR22247093.fastq.gz	Fastqsanger.gz
	SRR22247094.fastq.gz	Fastqsanger.gz

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Project description:Nasal administration of anti-CD3 monoclonal antibody (Foralumab) in COVID-19

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Project description:COVID-19 patients frequently develop neurological symptoms, but the biological underpinnings of these phenomena are unknown. Through single cell RNA-seq and cytokine analyses of CSF and blood from COVID-19 patients with neurological symptoms, we find compartmentalized, CNS specific T cell activation and B cell responses. All COVID-19 cases had CSF anti-SARS-CoV-2 antibodies whose target epitopes diverged from serum antibodies. In an animal model, we find that intrathecal SARS-CoV-2 antibodies are found only during brain infection, and are not elicited by pulmonary infection. We produced CSF-derived monoclonal antibodies from a COVID-19 patient, and find that these mAbs target both anti-viral and anti-neural antigens including one mAb that reacted to both spike protein and neural tissue. Overall, CSF IgG from 5/7 patients contains anti-neural reactivity. This immune survey reveals evidence of a compartmentalized immune response in the CNS of COVID-19 patients and suggests a role for autoimmunity in neurologic sequelae of COVID-19.

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