Project description:Endothelial cells (ECs) can detect oxygen and nutrient shortages in tissues and are able to adapt by inducing proliferation and migration into ischemic areas. Here, we present results that elucidate the striking role of a novel, EC/mural cell-specific gene, Apold1, in regulating pathological angiogenesis in ischemia. Screening of the transcriptomic profile by bulk RNAseq of wild type (WT) and Apold1-/- isolated muscle endothelial cells (ECs) revealed minor transcriptional differences upon Apold1 knockout. In contrast RNA sequencing on primary ECs derived from Apold1-/- or WT ECs, which were briefly (16 hrs) cultured in angiogenic medium, showed striking differences.
Project description:Single cell RNAseq from brain endothelial cells (BECs) isolated from wild-type and Iqgap2-/- (KO) mice to identify transcriptional changes in BECs caused by loss of Iqgap2. Mouse brains were dissociated to single cell suspension and labelled with CD31. CD31+ cells were flow sorted and processed for single cell RNAseq.