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Role of PARP-1 in transcriptional regulation during development

ABSTRACT: Role of PARP-1 in transcriptional regulation during development

PROVIDER: PRJNA900228 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22255509_1.fastq.gz	Fastqsanger.gz
	SRR22255509_2.fastq.gz	Fastqsanger.gz
	SRR22255510_1.fastq.gz	Fastqsanger.gz
	SRR22255510_2.fastq.gz	Fastqsanger.gz
	SRR22255511_1.fastq.gz	Fastqsanger.gz

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Role of PARP-1 in transcriptional regulation during development (ChIP-seq)

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Role of PARP-1 in transcriptional regulation during development

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CDK2-dependent activation of PARP-1 is required for hormonal gene regulation in breast cancer cells.

Project description:Eukaryotic gene regulation implies that transcription factors gain access to genomic information via poorly understood processes involving activation and targeting of kinases, histone-modifying enzymes, and chromatin remodelers to chromatin. Here we report that progestin gene regulation in breast cancer cells requires a rapid and transient increase in poly-(ADP)-ribose (PAR), accompanied by a dramatic decrease of cellular NAD that could have broad implications in cell physiology. This rapid increase in nuclear PARylation is mediated by activation of PAR polymerase PARP-1 as a result of phosphorylation by cyclin-dependent kinase CDK2. Hormone-dependent phosphorylation of PARP-1 by CDK2, within the catalytic domain, enhances its enzymatic capabilities. Activated PARP-1 contributes to the displacement of histone H1 and is essential for regulation of the majority of hormone-responsive genes and for the effect of progestins on cell cycle progression. Both global chromatin immunoprecipitation (ChIP) coupled with deep sequencing (ChIP-seq) and gene expression analysis show a strong overlap between PARP-1 and CDK2. Thus, progestin gene regulation involves a novel signaling pathway that connects CDK2-dependent activation of PARP-1 with histone H1 displacement. Given the multiplicity of PARP targets, this new pathway could be used for the pharmacological management of breast cancer. PARP-1 activation mechanism by CDK2 in response of progestin in breast cancer cells

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PARP-1 regulation of alternative splicing

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