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Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals [miRNA]

ABSTRACT: Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals [miRNA]

PROVIDER: PRJNA900352 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR22258620.fastq.gz	Fastqsanger.gz
	SRR22258621.fastq.gz	Fastqsanger.gz
	SRR22258622.fastq.gz	Fastqsanger.gz
	SRR22258623.fastq.gz	Fastqsanger.gz
	SRR22258624.fastq.gz	Fastqsanger.gz

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Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals [miRNA]

Project description:The right ventricular tissues of 5 children with tetralogy of Fallot and 5 healthy unaffected individuals were collected and sequenced in full transcriptome. Mechanism of tetralogy of fallot and cerna regulatory network were analyzed.

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mRNA expression profiling in patients with Tetralogy of Fallot and healthy unaffected individuals

Project description:Right ventricular mRNA profiles from 22 patients with Tetralogy of Fallot (TOF) and mRNA-seq profiles from the left and right ventricle (LV and RV, respectively) of 4 healthy unaffected individuals (NH) were generated. The total RNA was isolated from the 30 human heart samples using TRIzol. mRNAs were isolated from total RNA and prepared for sequencing using Illumina Kit RS-100-0801 according to the manufacturer's protocol (Preparing Samples for Sequencing of mRNA Sept 2008). The sequencing libraries were generated using a non-strand-specific library construction method. The purified DNA fragments were used directly for cluster generation, and 36 cycles of single-end read sequencing were performed using the Illumina Genome Analyzer. Sequencing reads were extracted from the image files using the open source Firecrest and Bustard applications (Illumina Genome Analyzer pipeline 1.3.2, 1.4.0 and 1.5.0). Right ventricular mRNA-seq profiles from 22 patients with Tetralogy of Fallot and mRNA-seq profiles from the left and right ventricle of 4 healthy unaffected individuals. Supplementary processed data files: GSE36761_gene_expression_levels.txt: Gene expression profiling data for all samples. GSE36761_gene_expression_levels_normalized.txt: Normalized gene expression profiling data for all samples. GSE36761_transcript_expression_levels.txt: Transcript expression profiling data for all samples. GSE36761_transcript_expression_levels_normalized.txt: Normalized transcript expression profiling data for all samples. Genome build: hg18

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Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals

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Small RNA sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals

Project description:Right ventricular small RNA profiles from 22 patients with Tetralogy of Fallot (TOF) and small RNA-seq profiles from the left and right ventricle (LV and RV, respectively) of 4 healthy unaffected individuals (NH) were generated. The total RNA was isolated from the 30 human heart samples using TRIzol. Small RNAs were isolated from total RNA and prepared for microRNA sequencing using Illumina Kit FG-102-1009 according to the manufacturer's protocol (Preparing Samples for Analysis of Small RNA Illumina 2007). The sequencing libraries were generated using a non-strand-specific library construction method. The purified DNA fragments were used directly for cluster generation, and 36 cycles of single-end read sequencing were performed using the Illumina Genome Analyzer. Sequencing reads were extracted from the image files using the open source Firecrest and Bustard applications (Illumina Genome Analyzer pipeline 1.3.2).

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Transcription profile in patients with acyanotic Tetralogy of Fallot

Project description:To determine cardiac transcription profile in acyanotic Tetralogy of Fallot patients, we collected myocardial samples immediately after institution of cardiopulmonary bypass from acyanotic Tetralogy of Fallot patients undergoing corrective surgery. The transcriptional profile of the mRNA in these samples was measured with gene array technology. Myocardial samples were collected, immediately after institution of cardiopulmonary bypass from acyanotic Tetralogy of Fallot patients undergoing corrective surgery.

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