Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA90055 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Determination of gene expression changes in cardiac muscle of mdx (dystrophin-deficient) mice at postnatal ages 8 weeks and 10.5 months. 3 independent replicates/age/strain.
2008-06-11 | E-GEOD-1471 | biostudies-arrayexpress
Project description:Determination of gene expression changes in cardiac muscle of mdx (dystrophin-deficient) mice at postnatal ages 8 weeks and 10.5 months. 3 independent replicates/age/strain. Keywords: parallel sample
2004-12-10 | GSE1471 | GEO
Project description:Rat muscle tissue group comparisons (skeletal-extraocular, skeletal-hindlimb, smooth-stomach, and cardiac Porter lab)
| PRJNA84535 | ENA
Project description:Extraocular muscle, comparison of wild type and mdx mice, 14 to 112 Days (Porter lab)
| PRJNA87063 | ENA
Project description:Extraocular and hindlimb muscle, comparison of wild type and mdx mice, 56 days (Porter lab)
| PRJNA90057 | ENA
Project description:Hindlimb muscle, comparison of wild type and mdx mice, 7 to 112 Day (Porter lab)
| PRJNA87093 | ENA
Project description:Extraocular, hindlimb, and cardiac muscles, comparison of dko and mdx mice (Porter lab)
| PRJNA90039 | ENA
Project description:Duchenne muscular dystrophy (DMD) is an incurable neuromuscular degenerative disease, caused by a mutation in the dystrophin gene. Mdx mice recapitulate DMD features. Here we show that injection of wild-type (WT) embryonic stem cells (ESCs) into mdx blastocysts produces mice with improved pathology. A small fraction of WT ESCs incorporates into the mdx mouse nonuniformly to upregulate protein levels of dystrophin in the skeletal muscle. The chimeric muscle shows reduced regeneration and restores dystrobrevin, a dystrophin-related protein, in areas with high and with low dystrophin content. WT ESC injection also normalizes the amount of fat, a tissue that does not express dystrophin. ESC injection without dystrophin does not prevent the appearance of phenotypes in the skeletal muscle or in the fat. Thus, dystrophin supplied by the ESCs reverses disease in mdx mice globally. Experiment Overall Design: 3-week old mdx (C57BL/10ScSn-Dmdmdx/J, Jax labs) females were superovulated and mated with mdx males (Jax labs). Blastocysts were collected at 3.5 days afer mating, injected with 15 WT or mdx R26 ES cells. Injected blastocysts were then transferred into the uteri of pseudopregnant females and allowed to develop to term. Skeletal muscle from 4 month old chimeric male mice was collected, RNA was isolated and microarray analysis were performed.
2008-10-25 | E-GEOD-12580 | biostudies-arrayexpress
Project description:In this study, in order to minimize the genetic variability of muscle samples we have used two approaches. First, we have analyzed the gene expression profile from a single skeletal muscle, the medial gastrocnemius (MG), and not from a pool of different muscles which could have different expression profiles. Second, we have performed the temporal gene expression profiling by extracting the MG muscles of the same individual from the both legs at two different times to minimize the inter-individual genetic variability. The MG muscle represent an excellent candidate for biopsy due to its easy accessible by surgery and also because its biopsy is well tolerated by the animals allowing us to perform another later biopsy in the other leg to obtain two MG samples of the same individual at two different times. Moreover, MG muscle is composed of approximately 20-30% type I (red) fibers and 70-80% type II (white) fibers {Ariano MA, 1973}, {Simard C, 1988}, {Zhan WZ, 1992} and therefore is more representative of skeletal muscle tissue in general than a muscle composed exclusively by red or white fibers.<br><br><br><br>The transcript expression profiles in MG muscles from mdx and wild-type mice were analyzed at 3 weeks, 1.5 months and 3 months of life by using the 430 2.0 gene chips from Affymetrix (n=3 for each condition). The differentially expressed transcripts which showed differences ?1.5-fold were obtained by performing three different comparisons: 1) genes differentially expressed in mdx compared with controls at each point in time (additional file 1); 2) temporal analysis of the genes differentially expressed in mdx mice between the three points in time also compared with the variations in control mice (additional file 2); and 3) temporal analysis of the genes differentially expressed in control mice between the three points in time also compared with the variations in mdx mice (additional file 3). The first comparison that we performed, by comparing the gene expression between mdx and control mice at every point in time, was similar to that performed in previous longitudinal studies {Porter JD, 2003}, {Rouger K, 2002}, {Turk R, 2005}. However, the other two comparisons were directed to elucidate the genes that are varying throughout the period of time analyzed in every mice strain, and therefore we obtained on the one hand the genes that vary in mdx mice but not in wild-type, and on the other hand the genes that vary in control animals but remain unchanged in mdx mice between the times analyzed. To present the results in a more comprehensive form, all the genes were classified in seven different categories: Cell adhesion & extracellular matrix; Proteolysis; Muscle structure & regeneration; Inflammation & immune response; Cell signaling & cell communication; Metabolism; and Others/unknown. The resulting genes from our study were classified in their functional categories using information from Affymetrix (www.affymetrix.com) and from the Gene Ontology database accessible in the Jackson Laboratory Mouse Genome Informatics website (www.informatics.jax.org).<br><br>
2009-04-16 | E-MEXP-1623 | biostudies-arrayexpress
Project description:Diaphram, comparison of wild type and mdx mice, 7 to 112 Days (Porter lab)
| PRJNA87095 | ENA