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Valine enhances intramuscular fat deposition through activating autophagy and fatty acid metabolism pathways in C2C12 myoblasts

ABSTRACT: Valine enhances intramuscular fat deposition through activating autophagy and fatty acid metabolism pathways in C2C12 myoblasts

PROVIDER: PRJNA900596 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22263676_1.fastq.gz	Fastqsanger.gz
	SRR22263676_2.fastq.gz	Fastqsanger.gz
	SRR22263677_1.fastq.gz	Fastqsanger.gz
	SRR22263677_2.fastq.gz	Fastqsanger.gz
	SRR22263678_1.fastq.gz	Fastqsanger.gz

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Valine enhances intramuscular fat deposition through activating autophagy and fatty acid metabolism pathways in C2C12 myoblasts

Project description:The branched-chain amino acids (BCAA) are essential to animal growth and intramuscular fat deposition, and also metabolic health (circulating level elevated in obesity and diabetes). However, the molecular role and effect of valine on muscle growth and fat deposition were less explored. Valine supplementation significantly affected mouse muscle growth, increasing the abdominal fat but decreasing the back fat. In the leg muscle, the expression levels of genes related to autophagy and fat deposition were significantly disturbed. Furthermore, valine promotes lipid deposition in myoblasts in a dose-dependent manner, and co-treatment of valine and palmitic acid can act in concert to enhance lipid deposition in myoblast. Moreover, transcriptome sequencing on myoblasts revealed that the signaling pathways such as mTOR, PI3K-AKT and fatty acid metabolism were activated after valine treatment, and palmitic acid additionally stimulated signaling pathways related to lipid metabolism in myoblasts. Therefore, valine could independently activate the autophagy pathway, and the fatty acid metabolism pathway to enhance intramuscular fat deposition

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circRNA expression in C2C12 myoblasts and myotubes

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lncRNA expression in C2C12 myoblasts and myotubes

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Project description:Maps of genomic regions in proximity to the nuclear lamina were determined in undifferentiated C2C12 myoblasts (MBs) and 6 day differentiated C2C12 myotubes (MTs) using DamID with a Dam-Lamin B1-encoding lentivirus.

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Genome-wide DNA methylation sequencing in C2C12 myoblasts and myotubes

Project description:We show the application of 5mC antibody-based methylated DNA immunoprecipitation followed sequencing technology for high-through profiling of DNA methylation in mouse C2C12 myoblasts and myotubes. By analyzing the methylation status of immunoprecipitated DNA fragments, we generated genome-wide DNA methyaltion maps in mouse C2C12 myoblasts and myotubes. We find that DNA methylation levels in myoblasts at rDNA promter and coding regions are higher than that in myotubes but not changed in intergenic regions.

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Project description:Proteosome inhibitors such as bortezomib (BTZ) have been used to treat muscle wasting in animal models. However, direct effect of BTZ on skeletal muscle cells has not been reported. In the present study, our data showed that C2C12 cells exhibited a dose-dependent decrease in cell viability in response to increasing concentrations of BTZ. We used microarrays to detail the differentially expressed genes in c2c12 myoblasts with different culture conditions.

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