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Single-cell RNA-seq of the mouse Peyer's patches blood endothelium

ABSTRACT: Single-cell RNA-seq of the mouse Peyer's patches blood endothelium

PROVIDER: PRJNA901180 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22270424.fastq.gz	Fastqsanger.gz
	SRR22270425.fastq.gz	Fastqsanger.gz
	SRR22270426.fastq.gz	Fastqsanger.gz
	SRR22270427.fastq.gz	Fastqsanger.gz
	SRR22270428.fastq.gz	Fastqsanger.gz

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Project description:To establish better understanding of cells found in jejunal and ileal Peyer's patches of pigs, we utilized single-cell RNA sequencing scRNA-seq and spatial transcriptomics to recover and analyze cells and spatial regions from sections of jejunum and ileum containing Peyer's patches. Cells identified via single-cell RNA sequencing included B, T/innate lymphoid cell, myeloid, epithelial, and stromal lineage cells. Spatial dots recovered via spatial transcriptomics belonged to regions including villi, crypts, interfollicular/parafollicular zones, follicles, and muscularis. Overall, results provide new information on regional localization and transcriptional profiles of cells in the pig small intestine.

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Expression data of ileal Peyer's patches from Salmonella Typhimurium infected pigs

Project description:This study couples a swine experimental model of non-typhoid salmonellosis to laser capture microdissection and microarray analysis to dissect the mechanisms carried out in the follicles of ileum Peyer’s Patches in response to Salmonella Typhimurium infection. Affymetrix GeneChip® Porcine genome array was used to study the gene expression profiles of Peyer's Patches grom control and infected pigs at acute phase of infection.

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