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RNAs bound by the glucocorticoid receptor during dexamethasone treatment

ABSTRACT: RNAs bound by the glucocorticoid receptor during dexamethasone treatment

PROVIDER: PRJNA901183 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22270510_1.fastq.gz	Fastqsanger.gz
	SRR22270510_2.fastq.gz	Fastqsanger.gz
	SRR22270511_1.fastq.gz	Fastqsanger.gz
	SRR22270511_2.fastq.gz	Fastqsanger.gz
	SRR22270512_1.fastq.gz	Fastqsanger.gz

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RNAs bound by the glucocorticoid receptor during dexamethasone treatment

Project description:The RNA binding ability of the glucocorticoid receptor (GR) remains an understudied area of GR regulation. Through in vitro binding assays, we identified hairpin RNAs as GR's preferred binding motif. To study how GR-bound RNAs change with dexamethasone treatment, we first generated stable U2OS cells expressing wild-type GR-HaloTag. We then treated the cells with dexamethsone every hour over three hours, UV crosslinked, harvested, and pulled down GR using a specific and covalently-bound HaloTag ligand. GR-bound RNAs were then isolated, library prepped, and sequenced.

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Effect of reduced glucocorticoid receptor-RNA affinity on dexamethasone treatment

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