Project description:In order to obtain genetically engineering strain of Penicillium oxalicum with high Raw Starch Digesting Glucoamylase production,we deleted PoxCxrC and overexpressed POX_f08097 in Penicillium oxalicum TE4-10 ,and we want to investigating the function of deletion of PoxCxrC and overexpression of POX_f08097 at transcriptional level.
Project description:The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), and Podot1 knockout strain (ΔPodot1) in different carbon sources. The deletion of Podot1 downregulated genes involved in the septin complex, extracellular region, and interspecies interaction between organisms when strains were cultivated with 2% glucose as carbon sources, and downregulated genes involved in cellulase activity, cellulose binding, glucosidase activity, and polysaccharide catabolic process when strains were cultivated with 1% microcrystalline cellulose and 1% wheat bran as carbon sources. We find the extracellular region was downregulated under both different carbon sources in ΔPodot1. This study provides the information that PoDot1 function are required in mycelial development and hydrolase activity of P. oxalicum.
