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Effects of RYGB on liver health in obese mice.

ABSTRACT: Effects of RYGB on liver health in obese mice.

PROVIDER: PRJNA901944 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22301132.fastq.gz	Fastqsanger.gz
	SRR22301133.fastq.gz	Fastqsanger.gz
	SRR22301134.fastq.gz	Fastqsanger.gz
	SRR22301135.fastq.gz	Fastqsanger.gz
	SRR22301136.fastq.gz	Fastqsanger.gz

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Similar Datasets

Effects of RYGB on liver health in obese mice.

Project description:We investigated the effects of RYGB compared to a sham surgery or caloric restriction on hepatic function. For that, the goals of this study is to identify changes in the liver transcriptome profiling (RNA-seq) that could explain improvements in liver health and function with RYGB compared to other treatment methods.

2024-03-06 | GSE218026 | GEO

mRNA Seq of mouse tissues 9 weeks after RYGB or sham surgery [9 weeks]

Project description:In this study we sought to characterize the long tern response to RYGB surgery using diet induced obese mice. Controls were sham operated mice. Mice were age, sex, weight and diet matched.

2018-07-09 | GSE113822 | GEO

Expression data from human jejunum before and one month after Roux en Y Gastric Bypass (RYGB)

Project description:RYGB reconfigures the intenstine such that food enters the jejunum after passing only a small gastric pouch. This new anatomy places the jejunum as the first part of the intestine that interacts with the food, with little to no gastric digestion, causing dramatic changes in jejunal morphology. Baseline is defined as the biopsy during RYGB. Patients are obese. We used microarrays to study the global programme of gene expression before and 1 month after RYGB, with biopsies taken from the same location the stomach-jejunum anastomosis.

2018-07-09 | GSE113819 | GEO

Liraglutide + PYY3-36 combination therapy mimics effects of Roux-en-Y bypass on early NAFLD whilst lacking-behind in metabolic improvements.

Project description:Background: Non-alcoholic fatty liver disease (NAFLD) is common in the general population. Treatment options for NAFLD are very limited, GLP-1 analogues, however, seem to be effective. Roux-en-Y gastric bypass (RYGB) is highly effective in terms of body weight loss and improves histologic and metabolic markers of NAFLD. We directly compared the effects of RYGB and a treatment with liraglutide and/or peptide tyrosine tyrosine 3-36 (PYY3-36) on early NAFLD. Methods: High-fat diet (HFD)-induced obese male Wistar rats were randomized into 6 treatment groups: RYGB, sham-operation (Sham), liraglutide (0.4mg/kg/day), PYY3-36 (0.1mg/kg/day), li-raglutide+PYY3-36 and saline. Following intervention and after an observation period of 4 weeks liver samples were histologically evaluated, ELISAs and RT-qPCRs +RNA sequencing were per-formed. Results: RYGB and liraglutide+PYY3-36 induced a similar body weight loss and marked histological improvements with significantly less steatosis compared to sham/saline. However, only RYGB induced significant metabolic improvements and mRNA expression changes. Con-clusions: liraglutide+PYY3-36 combination therapy mimics the positive effects of RYGB on weight reduction and on hepatic steatosis, while its effects on insulin resistance and adipose tissue dys-function (adiponectin/leptin ratio) lack behind RYGB.

2022-02-16 | GSE192425 | GEO

mRNA Seq of mouse tissues 9 days or 9 weeks after RYGB or sham surgery [9 days]

Project description:In this study we sought to characterize the acute response to RYGB surgery using diet induced obese mice. Controls were sham operated mice. Mice were age, sex, weight and diet matched.

2018-07-09 | GSE113821 | GEO

Effects of plantain on transcriptional profile of liver in obese rats

Project description:Effects of plantain on transcriptional profile of liver in obese rats

| PRJNA879729 | ENA

Evaluation of the physiological effects of a biotechnologically produced oyster mushroom in the liver of obese Zucker rats by transcriptomics

Project description:The hypothesis was tested that biotechnologically produced Pleurotus sajor-caju (PSC) influences metabolic health of obese Zucker rats. To test this, obese Zucker rats were randomly assigned to 2 groups of 12 rats each with similar body weights (BW) and fed diets either without (OC) or with 5 % PSC (OPSC) for 4 weeks and hepatic transcript profiling was performed. Transcriptomics of the liver revealed a total of 580 transcripts differentially expressed between OPSC and OC.

2020-06-06 | GSE151882 | GEO

Intestinal microbiota after RYGB

Project description:Objective: Roux-Y gastric bypass (RYGB) surgery is a last treatment resort to induce substantial and sustained weight loss in severe obesity. The anatomical rearrangement affects the intestinal microbiota but so far, little information is available how it interferes with microbial functionality and microbial-host interaction independent from weight loss. Design: A RYGB rat model was utilized and compared to sham-operated controls which were kept at matched body weight as RYGB animals by food restriction. We assessed microbial taxonomy by 16S rRNA gene sequencing and functional activity by metaproteomics and metabolomics on microbiota samples collected separately from the ileum, the cecum as well as the colon and separately analysed the lumen and mucus associated microbiota. Results: Altered gut architecture in RYGB strongly affected the occurrence of Actinobacteria, especially Bifidobacteriaceae and Proteobacteria which were increased, whereas Firmicutes were decreased, although Streptococcaceae and Clostridium perfringens were observed at higher abundances. A decrease of conjugated as well as secondary bile acids was observed in the RYGB-gut lumen. In addition the arginine biosynthesis pathway in the microbiota was altered, indicated by the changes in abundance of upstream metabolites and enzymes, resulting in lower levels of arginine and higher levels of aspartate in the colon after RYGB. Conclusion: The anatomical rearrangement in RYGB affects microbiota composition and functionality by changes in amino acid and bile acid metabolism, independent of weight loss. The shift in microbiota taxonomic structure after RYGB may be mediated by the resulting change in composition of the bile acid pool in the gut lumen.

2020-03-17 | PXD013337 | Pride

Effects of plantain on transcriptional profile of liver in obese rats

Project description:We explored the mechanisms underlying the anti-obesity effect of plantain. Plantain flour was used to feed obese rats fed a high-fat diet. Six groups were set, viz., the normal control (NC), obese control (OC), low-dose (LD), middle-dose (MD), high-dose (HD), and positive control (PC). The differentially expressed genes in the liver, as well as the lipid and glucose metabolic pathways were investigated. Transcriptome analyses showed that the expression of the PPARα, CPT1A, AMPK, and PGC1α genes was upregulated in the plantain intervention groups. Conclusion: Some key genes in fatty acid and sugar metabolic pathways were regulated by the plantain diet, which probably led to the observed fat and weight loss effects.

2022-09-15 | GSE213188 | GEO

Roux-en-Y gastric bypass and caloric restriction but not gut hormone-based treatments profoundly impact the hypothalamic transcriptome in obese rats

Project description:Background: The hypothalamus is an important brain region for the regulation of energy balance. Roux-en-Y gastric bypass (RYGB) surgery and gut hormone-based treatments are known to reduce body weight, but their effects on hypothalamic gene expression and signaling pathways are poorly studied. Methods: Diet-induced obese male Wistar rats were randomized into the following groups: RYGB, sham-operation, sham + body weight-matched (BWM) to the RYGB group, osmotic minipump delivering PYY3-36 (0.1mg/kg/day), liraglutide s.c. (0.4mg/kg/day), PYY3-36+liraglutide and saline. All groups (except BWM) were kept on a free choice of high- and low-fat diets. Four weeks after interventions, hypothalami were collected for RNA-sequencing. Results: While rats in the RYGB, BWM and PYY3-36+liraglutide groups had comparable reductions in body weight, only RYGB and BWM treatment had a major impact on hypothalamic gene expression. In these groups, hypothalamic leptin receptor expression as well as the JAK–STAT, PI3K-Akt and AMPK signaling pathways were upregulated. No significant changes could be detected in PYY3-36+liraglutide, liraglutide and PYY treated groups. Conclusions: Despite causing similar body weight changes compared to RYGB and BWM, PYY3-36+liraglutide treatment does not impact hypothalamic gene expression. Whether this striking difference is favorable or unfavorable to metabolic health in the long term requires further investigation.

2022-01-14 | GSE190218 | GEO

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