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ABSTRACT: Draft genome sequence of Nothapodytes nimmoniana (Graham) Mabb.

PROVIDER: PRJNA902349 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22312231_1.fastq.gz	Fastqsanger.gz
	SRR22312231_2.fastq.gz	Fastqsanger.gz

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Nothapodytes nimmoniana

Project description:Nothapodytes nimmoniana overview

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Proteomic and metabolomic analysis of Nothapodytes nimmoniana (J. Graham) extracts treatment on HeLa cells

Project description:Nothapodytes nimmoniana is a natural source of camptothecin, a known anticancer drug. Stem and leaf extracts of Nothapodytes nimmoniana were studied to assess its in-vitro action on cancer progression events in a cervical cancer cell line, the HeLa. As a well-studied ideal model system, the HeLa has been chosen and the effects of extracts on cancer progression events have been traced out. HeLa cells and media samples were analyzed for differentially expressed proteins and metabolites respectively on RRLC-ESI-QTOFMS. The CBFA2T1, cysteine-rich protein 2-binding protein, Zinc finger protein 788, transcription factor RFX3 and angiomotin-like protein 1 were significantly expressed proteins while 3-Hydroxysuberic acid, Indole-3-carboxylic acid, N8-Acetylspermidine, L-Octanoylcarnitine were significantly expressed metabolites. Aminoacyl-tRNA biosynthesis, purine metabolism, and valine, leucine, arginine, proline metabolism were the most prominent pathways. The multi-omics approach has the greatest metabolites and proteins profiling potential hence was applied here to find signatures of N. nimmoniana extracts treatment of cervical cancer.

2020-04-07 | PXD017375 | Pride

Nothapodytes nimmoniana

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Nothapodytes nimmoniana

Project description:Nothapodytes nimmoniana Transcriptome or Gene expression

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Nothapodytes nimmoniana

Project description:Nothapodytes nimmoniana Transcriptome or Gene expression

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Project description:Transcriptome analysis of young and mature leaf of Nothapodytes nimmoniana (Graham) Mabb. for understanding camptothecin biosynthesis

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Project description:Transcriptome analysis of Nothapodytes nimmoniana (Graham) Mabb. (Icacinaceae), a plant producing the anti-cancer alkaloid, camptothecin

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Transcriptional Profile of Lactobacillus helveticus grown in milk vs. MRS medium

Project description:L. helveticus is used to modulate cheese flavor and as a starter organism in certain cheese varieties. Our group has compiled a draft (4x) sequence for the 2.4 Mb genome of an industrial strain L. helveticus CNRZ32. The primary aim was to investigate expression of 168 completely sequenced genes during growth in milk and MRS medium using microarrays. Oligonucleotide probes against each of the completely sequenced genes were compiled on maskless photolithography-based DNA microarrays. Additionally, the entire draft genome sequence was used to produce tiled microarrays where the non-interrupted sequence contigs were covered by consecutive 24-mer probes. Keywords: growth conditions response

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