Effects of triiodothyronine and/or Irisin on human subcutaneous adipocytes: a global transcriptome analysis
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ABSTRACT:
PROVIDER: PRJNA902379 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR22314348_1.fastq.gz | Fastqsanger.gz | |||
| SRR22314348_2.fastq.gz | Fastqsanger.gz | |||
| SRR22314349_1.fastq.gz | Fastqsanger.gz | |||
| SRR22314349_2.fastq.gz | Fastqsanger.gz | |||
| SRR22314350_1.fastq.gz | Fastqsanger.gz |
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Project description:In order to investigate, at the mRNA level, the signaling pathways through which triiodothyronine (T3) and irisin (I) affects Human subcutaneous adipocytes function, human Cultured Human subcutaneous preadipocytes were differentiated into mature adipocytes, which were cultured with T3 and/or Irisin, the control group was without treatment. RNA-Seq was performed using Illumina platform, and differential gene expression was assessed with DESeq2. Among differentially expressed genes, enrichment analysis was performed for biological processes against the Gene Ontology Consortium database, using both ClusterProfiler R package and STRING. Grant 2016/03242-3, São Paulo Research Foundation (FAPESP). Grant 409438/2016, National Council for Scientific and Technological Development (CNPq).
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Project description:Obesity is characterized by the excess of body fat leading to impaired health. Abdominal fat is particularly harmful and is associated with cardiovascular and metabolic diseases and cancer. In contrast, subcutaneous fat is generally considered less detrimental. The mechanisms that establish the cellular characteristics of these distinct fat types in humans are not fully understood. Here, we explored whether differences of their gene regulatory mechanisms can be investigated in vitro. For this purpose, we in vitro differentiated human visceral and subcutaneous pre-adipocytes into mature adipocytes and obtained their gene expression profiles and genome-wide H3K4me3, H3K9me3 and H3K27ac patterns. Subsequently, we compared those data with public gene expression data from visceral and subcutaneous fat tissues. We found that the in vitro differentiated adipocytes show significant differences in their transcriptional landscapes, which correlate with biological pathways that are characteristic for visceral and subcutaneous fat tissues, respectively. Unexpectedly, visceral adipocyte enhancers are rich on motifs for transcription factors involved in the Hippo-YAP pathway, cell growth and inflammation, which are not typically associated with adipocyte function. In contrast, enhancers of subcutaneous adipocytes show enrichment of motifs for common adipogenic transcription factors, such as C/EBP, NFI and PPARgamma, implicating substantially disparate gene regulatory networks in visceral and subcutaneous adipocytes. Consistent with the role in obesity, predominantly the histone modification pattern of visceral adipocytes is linked to obesity-associated diseases. Thus, this work suggests that the properties of visceral and subcutaneous fat tissues can be studied in vitro and provides preliminary insights into their gene regulatory processes.
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