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Macrophage-CITED2 and inflammatory gene expression

ABSTRACT: Macrophage-CITED2 and inflammatory gene expression

PROVIDER: PRJNA902754 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22322067_1.fastq.gz	Fastqsanger.gz
	SRR22322067_2.fastq.gz	Fastqsanger.gz
	SRR22322068_1.fastq.gz	Fastqsanger.gz
	SRR22322068_2.fastq.gz	Fastqsanger.gz
	SRR22322069_1.fastq.gz	Fastqsanger.gz

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Macrophage-CITED2 and inflammatory gene expression

Project description:CITED2 is a member of the CBP/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain family of transcriptional regulators and is exclusively localized in the nucleus. Previous studies have demonstrated that CITED2 plays an important role in cellular development and differentiation. Experimental murine studies have shown that Cited2 mutation or deficiency results in congenital heart and neural crest defects, impairment in the development of lung, liver, placenta, and adrenal tissue, and perturbation in the left-right patterning of the body axis. Previous studies have also show that CITED2 is predominantly expressed in murine and human macrophages. Myeloid-specific CITED2 deficient mice are highly susceptible to lipopolysaccharide (LPS)-induced endotoxic shock syndrome, zymosan-induced lung inflammation, and experimental atherogenesis. Mechanistically, CITED2 in cooperation with PPARγ promotes anti-inflammatory gene expression while suppressing NFκB, HIF1α, STAT1, and IRF1 signaling to attenuate pro-inflammatory gene expression in macrophages.

2022-12-08 | GSE218217 | GEO

Role of CITED2 in shaping macrophage inflammatory response

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Role of CITED2 in shaping macrophage inflammatory response

Project description:CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxyl-terminal domain 2 (CITED2) is a member of the CITED family of proteins that play very critical roles in cellular development and differentiation. Several independent studies have revealed that mutation in the CITED2 gene is associated with human congenital heart defects. Concordant with these observations, experimental murine studies affirmed that mutation or loss of Cited2 resulted in congenital heart defects, as well as perturbation in the left-right patterning of the body axis. Furthermore, deficiency of Cited2 significantly impaired the development of lung, liver, placenta, and adrenal tissue at the embryonic stage (PMCID: PMC5809687).

2020-09-23 | GSE147943 | GEO

Identification of CITED2 target genes in human CB

2014-09-05 | GSE47218 | GEO

Identification of CITED2 target genes in human CB

Project description:CB CD34+ cells were were transduced with control (tNGFR) or CITED2 overexpression lentivectors, or control (shSCR) or shRNA CITED2 lentivectors and mRNA was isolated in order to investigate global gene expression changes upon perturbation of CITED2. CB CD34+ cells were isolated by Miltenyi miniMACS column. Cells were prestimulated in HPGM with 100 ng/ml KITL, FLT3L and TPO for 12 hrs. Cells were transduced with control (tNGFR) or CITED2 overexpression lentivectors, or control (shSCR) shRNA CITED2 lentivectors, in three rounds over 48 hrs. Transduced cells were sorted after which RNA was isolated for Illumina beadhchip arrays HT12 v4.

2014-09-05 | E-GEOD-47218 | biostudies-arrayexpress

Aberrant expression of CITED2 promotes prostate cancer metastasis by activating the nuceolin-AKT pathway.

Project description:We found that CITED2 is highly expressed in metastatic prostate cancer, and its expression is correlated with poor survival in pateints. In this study, we used an siRNA to decrease CITED2 expression in PC3 cells. A RNA-seq approach was utilized in order to determine global gene expression changes in CITED2 knockdown cells compared to control cells.

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Effect of Cited2 knockout on gene expression in haematopoietic stem cells

Project description:The pleiotropic transcriptional regulator CITED2 is essential for lifelong maintenance of haematopoiesis. To understand the molecular roles of CITED2 in normal haematopoiesis, we performed RNA-sequencing on CD48−CD150+ haematopoietic stem cells (HSCs) derived from young mice.

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Gene expression analysis in CITED2 knockdown MDA-MB-231 human breast cancer cells.

Project description:The goal of this experiment is to explore the mechanism behind CITED2 (a non-DNA binding transcriptional co-activator) metastatic action by comparing gene expression changes between shScramble and shCITED2-expressing MDA-MB-231 cells.

2017-01-14 | E-MTAB-4267 | biostudies-arrayexpress

Effects of a high-fat maternal diet and embryonic Cited2 genotype on gene expression and development in mouse

Project description:Deficiency of the transcription factor Cited2 in mice results in cardiac malformation, adrenal agenesis, neural tube, placental defects, and partially penetrant left-right patterning defects resulting from an abnormal Nodal->Pitx2c pathway. Here we show that a maternal high-fat diet more than doubles the penetrance of laterality defects and, surprisingly, induces palatal clefting in Cited2 deficient embryos. Both maternal diet and Cited2 deletion reduce embryo weight and kidney and thymus volume. Expression profiling identified 40 embryonic transcripts including Pitx2 that were significantly affected by embryonic genotype-maternal diet interaction. We show that a high-fat diet reduces Pitx2c levels >2 fold in Cited2 deficient embryos and that Pitx2c deficiency results in cleft palate. Taken together, these results define a novel interaction between maternal high-fat diet and embryonic Cited2 deficiency that affects Pitx2c expression and results in abnormal laterality and cleft palate.

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Project description:Effect of Cited2 knockout on gene expression in haematopoietic stem cells

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