Project description:We report the proteomic characterization of gonads from wild P. lividus collected along coastal Sardinia, and describe the changes occurring in gonads according to sex and developmental stage. Gonads in the recovery, pre-mature, mature, and spent stages were analyzed using a shotgun proteomics approach based on filter-aided sample preparation followed by tandem mass spectrometry and label-free differential analysis. A detailed characterization of the proteome changes occurring in gonads of both sexes along maturation was achieved. Significant changes were seen in numerous proteins involved in nutrient accumulation and in gamete biology and maturation. Adding to an improved understanding of the P. lividus reproductive cycle in its natural environment, the results described in this work form the basis for defining novel protein markers and procedures for an easier sexing and staging, and for monitoring sea urchin gonad maturation in aquaculture plants.
