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Effects of m6A modification and YTHDF2 knockdown on global mRNA turnover

ABSTRACT: Effects of m6A modification and YTHDF2 knockdown on global mRNA turnover

PROVIDER: PRJNA902825 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22326400_1.fastq.gz	Fastqsanger.gz
	SRR22326400_2.fastq.gz	Fastqsanger.gz
	SRR22326401_1.fastq.gz	Fastqsanger.gz
	SRR22326401_2.fastq.gz	Fastqsanger.gz
	SRR22326402_1.fastq.gz	Fastqsanger.gz

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Project description:Our study demonstrated that the expression of Igf2bp1 in activated microglia was significantly up-regulated, implying a role of Igf2bp1 in LPS-induced m6A modifications in microglia. To understand the roles of Igf2bp1 on LPS-induced m6A modification in microglia, we performed Igf2bp1 loss-of-function (LOF) approach. Microglia stimulated by LPS were transfected with either scrambled siRNA control or Igf2bp1 siRNA for 48 hours. To m6A modification profiles in control and Igf2bp1 LOF microglia were determined by MeRIP-seq analysis.

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