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SCOPE-seq Analysis of Intra-operative Labeling Specificity of 5-ALA for Glioblastoma

ABSTRACT: SCOPE-seq Analysis of Intra-operative Labeling Specificity of 5-ALA for Glioblastoma

PROVIDER: PRJNA903155 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22334917_1.fastq.gz	Fastqsanger.gz
	SRR22334917_2.fastq.gz	Fastqsanger.gz
	SRR22334918_1.fastq.gz	Fastqsanger.gz
	SRR22334918_2.fastq.gz	Fastqsanger.gz
	SRR22334919_1.fastq.gz	Fastqsanger.gz

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SCOPE-seq Analysis of Intra-operative Labeling Specificity of 5-ALA for Glioblastoma

Project description:Glioblastoma (GBM) is the most common and aggressive malignant primary brain tumor, and surgical resection is a key part of the standard-of-care. In fluorescence-guided surgery (FGS), fluorophores are used to differentiate tumor tissue from surrounding normal brain. The heme synthesis pathway converts 5-aminolevulinic acid (5-ALA), a fluorogenic substrate, to the fluorophore protoporphyrin IX (PpIX). The resulting fluorescence is thought to be specific to transformed glioma cells, but this specificity has not been examined at single cell level. Here, we performed paired single cell imaging and RNA sequencing of individual cells (SCOPE-seq2) on human GBM surgical specimens with visible PpIX fluorescence from patients who received 5-ALA prior to surgery. SCOPE-seq2 allows us to simultaneously measure PpIX fluorescence by imaging and unambiguously identify transformed glioma cells from single-cell RNA-seq (scRNA-seq). We observed that 5-ALA treatment results in labeling that is not specific to transformed tumor cells. In cell culture experiments, we further demonstrated untransformed cells can be labeled by 5-ALA directly or by PpIX secreted from surrounding transformed cells. In acute slice cultures from mouse glioma models, we showed that 5-ALA preferably labels GBM tumor tissue over non-neoplastic brain tissue at bulk level, and that this contrast is not due to blood-brain-barrier disruption. Taken together, our findings support the use of 5-ALA as an indicator of GBM tissue, but not as a specific marker of transformed glioma cells.

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MESO-SCOPE, SCOPE cruise July 2017

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Project description:We aim to provide the very first insight into ALA+MM molecular profiles and compare the results with ALA and with MM. Our detailed study of ALA and ALA+MM represents an important step towards improved understanding of their genetic and transcriptomic background, which is a prerequisite for development of optimal treatment strategies in the future.

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ALA-CPM

Project description:Interventions: 3-4 hours before the operation, 1.5 g (20 mg / kg) of araglio granules was dissolved in 50 ml of water and orally administered. Under general anesthesia and insufflation of carbon dioxide gas, the inside of the abdominal cavity is observed using a white and fluorescent light source device. The presence or absence of fluorescence is evaluated using normal light (white light) and blue light.;Oral administration of 5-ALA Primary outcome(s): ALA-PDD increases the accuracy of peritoneal metastasis (Comparison of sensitivity between white light and blue light) Study Design: single arm study, open(masking not used), uncontrolled control, single assignment, diagnostic purpose

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Project description:We report the application of 5-ALA ( 5-Aminolevulinic acid)-mediated photodynamic therapy on mouse brain tissue, and further explored the impact of PDT on nervous system.

2021-04-30 | GSE173615 | GEO

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