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Bulk RNA-seq expression data isolated from early L1 and L4 stage C. elegans larvae

ABSTRACT: Bulk RNA-seq expression data isolated from early L1 and L4 stage C. elegans larvae

PROVIDER: PRJNA903188 | ENA |

REPOSITORIES: ENA

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Bulk RNA-seq expression data isolated from early L4 stage C. elegans larvae

Project description:During development C. elegans GABAergic dorsal D-class (DD) motor neurons reverse polarity such that the axonal and dendritic compartments are switched. We found that the gene DVE-1 is important for this process. To elucidate potential targets of dve-1 transcriptional regulation we conducted bulk RNA sequencing of total RNA from L4 stage wild type and dve-1 mutant animals

2024-12-08 | GSE218350 | GEO

Bulk RNA-seq expression data isolated from early L4 stage C. elegans larvae

Project description:Bulk RNA-seq expression data isolated from early L4 stage C. elegans larvae

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Sequencing of Caenorhabditis elegans wildtype strain (N2) treated with T25B9.1 RNAi for 5 days after L4 larvae stage.

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Bulk RNA-seq expression data isolated from early L1 stage C. elegans larvae

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Bulk RNA-seq expression data isolated from early L1 stage C. elegans larvae

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Comparing the transcriptomic responses of Caenorhabditis elegans exposed to cadmium from L1 to L3 versus L1 to L4 stage

Project description:Cadmium is a naturally occurring heavy metal, and it is widely used in industry. Due to its persistent toxic effect, cadmium is classified as a category one human carcinogen. Its toxicity has been extensively studied in different organisms, including the nematode Caenorhabditis elegans. To investigate the transcriptomic responses following cadmium during early life exposure, C. elegans larval were exposed either from L1 to L3 stage or L1 to L4 stage to 20 µM cadmium chloride. RNAseq was applied to identified the changes of the transcriptome and the pathways which are specific to each stage. Overall, the result revealed that the highest responsive genes in both exposure scenario were linked to oxidative stress, lipid metabolism and ion binding. Additionally, some of these genes are well characterized and linked to specific functions, but some have no known function, however these uncharacterized genes are differentially expressed in both stages as a result of cadmium toxicity.Numerous transcripts were identified in one stage but not at the other. Furthermore, a general trend was observed where a larger number of genes were differentially expressed at L3 stage compared to L4, which suggest stage specific sensitivity in response to cadmium exposure.

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Expression data from DD neurons isolated from early L1 stage C. elegans larvae.

Project description:Six DD class GABAergic neurons are generated in the embryo to synapse with ventral muscles and receive input from cholinergic neurons in the dorsal nerve cord. After hatching and toward the end of the first larval (L1) stage, DD neurons reverse polarity (i.e., synapse with dorsal muscles, receive ventral cholinergic inputs). Expression profiles were generated from DD neurons in the early L1 stage before the initiation of the remodeling program. We used microarray analysis to detect transcripts with potential roles in DD remodeling. We used FACS to isolate ttr-39::mCherry labeled DD GABAergic motor neurons from a synchronized population of L1 larvae and amplified and labeled total RNA to generate Affymetrix Genome Array data. The DD data sets were compared to an expression profile obtained from all cells in a matched population of synchronized L1 larvae. See Spencer et al. PLOS One 9, e112102 (2014).

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