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Homo sapiens cultivar:HaCaT cell

ABSTRACT: Comparative transcriptome profiles of human HaCaT cells in response to three different extracts of Gynostemma pentaphyllum by RNA sequencing

PROVIDER: PRJNA903215 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22342813_1.fastq.gz	Fastqsanger.gz
	SRR22342813_2.fastq.gz	Fastqsanger.gz
	SRR22342814_1.fastq.gz	Fastqsanger.gz
	SRR22342814_2.fastq.gz	Fastqsanger.gz
	SRR22342815_1.fastq.gz	Fastqsanger.gz

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Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) between HaCaT cell and OSM-treated HaCaT cells Methods: mRNA profiles of HaCaT cells and OSM-treated HaCaT cells were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.

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