Bandavirus heartlandense
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA904354 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Heartland virus Genome sequencing and assembly
| PRJNA742388 | ENA
Project description:This study is to determine and compare the transcriptomes in two eukaryotic hosts (mammalian host - DH82 canine cell line and tick vector - ISE6 Ixodes scapularis cell line) of eight Ehrlichia chaffeensis strains in three divergent genetic groups, including Arkansas, Heartland, Jax, Liberty, Osceola, St. Vincent, Wakulla, and West Paces, and one Ehrlichia sp. HF strain.
2014-04-01 | GSE56339 | GEO
Project description:This study is to determine and compare the transcriptomes in two eukaryotic hosts (mammalian host - DH82 canine cell line and tick vector - ISE6 Ixodes scapularis cell line) of eight Ehrlichia chaffeensis strains in three divergent genetic groups, including Arkansas, Heartland, Jax, Liberty, Osceola, St. Vincent, Wakulla, and West Paces, and one Ehrlichia sp. HF strain. RNA samples for the Arkansas (reference), Heartland, Jax, Liberty, Osceola, St. Vincent, Wakulla, and West Paces strains in both tick and canine cell lines, including two uninfected controls, will be isolated in triplicate. As such 108 libraries will be constructed and sequenced corresponding to (8 strains + 1 control) x 2 conditions (tick cells + dog cells) x 3 biological triplicates x 2 molecules sequenced (eukaryotic RNA + prokaryotic RNA). The transcriptomes are obtained with Illumina HiSeq reads obtained from paired end libraries. The bacterial reads will be mapped against the reference genome and compared through a differential expression analysis. The eukaryotic reads from the same cell types (i.e. tick or canine) will be assembled with Trinity. The reads will then be mapped back to this assembly for the differential expression analysis.
2014-04-01 | E-GEOD-56339 | biostudies-arrayexpress
Project description:Molecular phylogenetics project (Palmblad et al. 2011)
2012-01-11 | PRD000375 | Pride