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Effect of long-term ethanol treatment in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)

ABSTRACT: Effect of long-term ethanol treatment in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)

PROVIDER: PRJNA904392 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22383476_1.fastq.gz	Fastqsanger.gz
	SRR22383476_2.fastq.gz	Fastqsanger.gz
	SRR22383477_1.fastq.gz	Fastqsanger.gz
	SRR22383477_2.fastq.gz	Fastqsanger.gz
	SRR22383478_1.fastq.gz	Fastqsanger.gz

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Chronic ethanol exposure induces deleterious changes in cardiomyocytes derived from human induced pluripotent stem cells

Project description:Proteomic analyssis of hiPSC-CMs treated with long-term ethanol

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Global gene expression profiles of cardiomyocytes differentiated from human pluripotent stem cells (hiPSC-CMs) in 3D culture exposed to ethanol

Project description:Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq2500 system. Reads were mapped on the Human Genome Reference and normalized expression table was generated. Results: RNA-seq results reveal gene expression of cardiac toxicity in hiPSC-CMs that are consistent with alcohol-induced pathophysiology observed in animal models. For example MMP9 is among the top 5 upregulated genes in ethanol-treated hiPSC-CMs, MMP9 concentrations are significantly higher in human sera of chronic alcohol abusers and MMP9 mRNA and protein levels are increased in the myocardium of rats following acute ethanol exposure. Conclusions: Data demonstrate significant alteration in gene expression, among the top 60 genes significantly altered by ethanol exposure, 8 genes are involved in ion channels, which may be in part contributing to the abnormal intracellular Ca2+ transients. Ethanol up-regulated the expression of genes associated with collagen metabolism and extracellular matrix (MMP9, EMID1, COL14A1), most of the downregulated genes are involved in cardiovascular system development (NPPB, DNAAF3), actin filament-based process (LMOD2, MYH4) and muscle contraction (MYL2). These findings are consistent with previous studies showing a correlation between alcohol exposure and defects in heart and circulatory system development.

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microRNA profile expression analysis of Human Induced Pluripotent Stem Cell-derived cardiomyocytes (hiPSC-CMs)

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Effect of long-term ethanol treatment in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)

Project description:We treated our iPSC-CMs with EtOH for 30+ days to investigate changes in gene expression

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RNA-Seq transcriptome profiling of human iPS cell-derived cardiomyocytes (hiPSC-CMs) following overexpression (OE) of estrogen-related receptor gamma (ERRg)

Project description:Transcriptional regulatory circuits that drive cardiomyocyte maturation are poorly understood. Human iPS cell-derived cardiomyocytes (hiPSC-CMs) have been shown to have fetal cardiomyocyte features in terms of metabolic gene expression profiles. Here we found that in hiPSC-CMs, overexpression of estrogen-related receptor gamma (ERRg) is sufficient to drive cardiomyocyte metabolic maturation programs including the induction of a number of oxidative mitochondrial metabolic genes.

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Altered drug response of recovered human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from cryopreservation

Project description:We conducted RNA sequcing of the fresh and 1-week recoverd human induced pluripotent stem cell-derived caridomyocytes (hiPSC-CMs) undergo cryopreservation

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RNA sequencing of wild-type and RBPMS2-null human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)

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Transcriptome analysis of hiPSC-CMs carrying FLNC mutations

Project description:In this study we performed transcriptome analysis of cardiomyocytes (CMs) derived from two hiPSC lines of patients with V2264M and R1267Q mutations in FLNC gene. hiPSC cell line were obtained from patient’s PBMCs with FLNC mutation using CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific). Detailed information about iPSC line characterization can be found in hiPSCreg database: (FAMRCi011-A and FAMRCi009). Information about FAMRCi009 is earlier published DOI: 10.1016/j.scr.2021.102640.

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