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Rapid Acceleration of Diagnostics - Underserved Populations (RADx-UP):C2SPARC: Implementing Mobile, POC COVID-19 Testing in Partnership with a Community-based Organization to Reach Women Who Use Drugs

ABSTRACT: Rapid Acceleration of Diagnostics - Underserved Populations (RADx-UP):C2SPARC: Implementing Mobile, POC COVID-19 Testing in Partnership with a Community-based Organization to Reach Women Who Use Drugs

PROVIDER: PRJNA904482 | ENA |

REPOSITORIES: ENA

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Rapid Acceleration of Diagnostics - Underserved Populations (RADx-UP):C2SPARC: Implementing Mobile, POC COVID-19 Testing in Partnership with a Community-based Organization to Reach Women Who Use Drugs

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Project description:Small RNAs recently emerged as a new class of mobile instructive signals in development. Here, we investigate their mechanism of action and show that the gradients formed by mobile small RNAs generate sharply defined domains of target gene expression. By modulating the source of artificial miRNAs we show that boundary formation is an inherent property of the small RNA gradient itself. The threshold-based readout of such gradients is highly sensitive to small RNA levels at the source, allowing plasticity in the positioning of a target gene expression boundary. In addition to generating sharp expression domains of their immediate targets, the readouts of opposing small RNA gradients enable formation of stable and uniformly positioned developmental boundaries. These novel patterning properties of small RNAs are reminiscent of those of morphogens in animal systems. However, their exceptionally high specificity, direct mode of action, and the fully intrinsic nature of their gradients, distinguish mobile small RNAs from classical morphogens. Our findings present mobile small RNAs and their targets as highly portable and evolutionarily-tractable regulatory modules through which to create pattern in development and beyond.

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Acetomicrobium mobile DSM 13181

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Knockdown specificity of Mobile-CRISPRi system targeting chromosomal mRFP

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