Project description:To analyze wha's the function of anti-FGl2-scFv on T cells, sorted CD8+T cells transfected with empty vector or with anti-FGL2-scFv were processed RNA next generation sequencing
Project description:In order to study heme-induced global transcriptional changes, we performed a transcriptome-wide analysis of heme influence on the A. aegypti cell line Aag2. As heme-mediated effects on gene expression are thought to be related to oxidative stress, we compared the transcriptional profiles of Aag2 cells challenged with either 50 µM heme or 100 µM of the ROS inducer paraquat, using whole genome microarrays. Three-condition experiment, CTR vs. Heme- or Paraquat incubated cells. Biological replicates: 4 control and 4 experimental replicates.
Project description:Bulk RNAseq data looking at transcriptional differences between Mtb-specific T cells in the lung, vs those in the vasculature (with naive, CD44low controls) during Mycobacterium tuberculosis infection. Allowing us an unbiased way to discern differentially expressed genes in the two tissue spaces.
Project description:Macophage migration inhibitor (MIF) is important for MM resistance to proteasome inhibitors. To determine what signalling pathways are affected by MIF in MM cells, we established MIF-KO MM.1S and ARP-1 MM cell lines. Total RNAs of 2 x 106 CTR-KO and MIF-KO MM cells were extracted by RNeasy Mini Kit (Qiagen). 5-10 µg RNA samples were sent to Gene Expression and Genotyping Facility at Case Western Reserve University (Cleveland, OH) for RNA-seq followed by data analysis. We use microarray data to determine differential expression of genes in MIF-KO and CTR-KO MM cells.
