Project description:Mandarin fish Siniperca chuatsi (Basilewsky) (Percichthyidae), as a demersal piscivore, has very specialized feeding habits, for as soon as they start feeding the fry of this fish feed solely on fry of other fish species. In rearing conditions, mandarin fish has been found to accept live prey fish only, and refuse dead prey fish or artificial diets, very little is currently known about the molecular mechanisms of multiple genes which cover different pathways influencing the specialized food habit, such as live prey. We performed transcriptome comparisons between dead prey fish feeders and nonfeeders in mandarin fish. The determination mechanisms of specialized food habit (live prey fish) in mandarin fish could provide some instructions for research of food habit in animals, including mammals.
Project description:To characterize the site-specific methylation landscape of the Mandarin fish ranavirus (MRV) genome, whole-genome bisulfite sequencing (WGBS) was conducted on an isolated MRV strain.
Project description:Proteomics sequence of ricefield eel gonads at five different development stages, the ovary (OV), early intersexual stage gonad (IE), middle intersexual stage gonad (IM), late intersexual stage gonad (IL), and testis (TE).
Project description:Growth and reproduction are two major physiological processes that are closely related in vertebrates, and IGFs are key factors that regulate the growth and reproduction axis. As a novel IGF system member, igf3 plays an important role in gonadal development of teleost fish. Although studies have reported the abnormal expression of igf3 in fish gonad, whether the igf3 regulates the expression of long noncoding RNAs (lncRNAs) in gonad remains unknown. In this study, a igf3 knockdown common carp (Cyprinus carpio) model was established by RNA interference. Than RNA sequencing of transcriptome in C. carpio after igf3 knockdown was performed. A total of 327,169,410 and 306,305,018 clean reads were identified from control and igf3-dsRNA group, respectively. After a stringent filtering, RNA-seq yielded 14199 lncRNA and 106932 mRNA transcripts with 124 and 353 differentially expressed lncRNAs and mRNAs. Gene Ontology analysis revealed that the target genes of differentially expressed lncRNAs and mRNAs mainly regulated male genitalia development, female gonad development, cellular response to gonadotropin-releasing hormone, insulin-like growth factor-activated receptor activity and insulin-like growth factor binding. Kyoto Encyclopedia of Genes and Genomes analysis showed that the target genes of differentially expressed lncRNAs and mRNAs mainly involved in pathways associated with insulin signaling pathway, GnRH signaling pathway, Wnt signaling pathway, PI3K-Akt signaling pathway and TGF-beta signaling pathway. The lncRNA-mRNA co-expression network highlight the inter-regulation of the differentially expressed lncRNAs and mRNAs in gonad. Our results provide essential data regarding differentially expressed lncRNAs and mRNAs after igf3 knockdown, which may be useful to elucidate the role of igf3 in the gonadal development of C. carpio.
2018-10-27 | GSE121692 | GEO
Project description:Transcriptome analysis of the growth performance of domesticated hybrid mandarin fish
| PRJNA610675 | ENA
Project description:Trancriptome sequencing in mandarin fish
Project description:Sexual reproduction is a complex process involving multiple pathways. In this study, we aimed at investigating gene expression profiles underlying gender specificity in the zebrafish. To do so, we analyzed the transcriptome of the gonads of breeding fish and queried the gene expression profiles for significant differences between the genders and also between stages of gonad development. Major differences in gene expression profiles between genders were encountered at the level of the gonads. In addition, we identified biological processes associated with stage of sexual development in female gonads. Keywords: gender and stage related gene expression