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The rDNA antisense transcript PAPAS promotes lactogenic differentiation of mammary epithelial cells and suppresses breast cancer

ABSTRACT: The rDNA antisense transcript PAPAS promotes lactogenic differentiation of mammary epithelial cells and suppresses breast cancer

PROVIDER: PRJNA905350 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22407600_1.fastq.gz	Fastqsanger.gz
	SRR22407600_2.fastq.gz	Fastqsanger.gz
	SRR22407601_1.fastq.gz	Fastqsanger.gz
	SRR22407601_2.fastq.gz	Fastqsanger.gz
	SRR22407602_1.fastq.gz	Fastqsanger.gz

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The rDNA antisense transcript PAPAS promotes lactogenic differentiation of mammary epithelial cells and suppresses breast cancer

Project description:PAPAS is an antisense transcript of rRNA genes and mediates silencing of the locus in non-dividing cells. Here, we have analyzed PAPAS function in lactogenic differentiation of mammary epithelial cells (HC11 cells) and in mammary cancer cells (4T1). Using doxycycline (Dox) inducible shRNA against PAPAS, we found that the knockdown perturbs differentiation and inhibits expression of lactogenic genes. In contrast, Dox-inducible overexpression of PAPAS in 4T1 cells suppresses genes required for ribosome biogenesis and slows down the neoplastic growth.

2024-01-01 | GSE218723 | GEO

Expression of Mouse and Human Sense-Antisense Transcript

Project description:Expression of Mouse and Human Sense-Antisense Transcript

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Knockdown of PAPGEF3-antisense transcript

Project description:We performed siRNA-mediated knockdown of noncoding RAPGEF3-antisense transcript. Experiment were done with six repeat of each treatment

2010-11-03 | E-MTAB-419 | biostudies-arrayexpress

A natural antisense transcript of notch-1 regulates cell cycle

Project description:Here we demonstrate a natural antisense transcript of notch-1 that interacts with the sense transcript and regulates its availability and pace of G1 phase of cell cycle.

2021-03-03 | GSE168092 | GEO

Zinc-dependent regulation of the adh1 antisense transcript in fission yeast

Project description:In yeast, alcohol dehydrogenase I (Adh1) is an abundant zinc binding protein that is required for the conversion of acetaldehyde to ethanol. Through transcriptome profiling of the Schizosaccharomyces pombe genome, we identified a natural antisense transcript at the adh1 locus that is induced in response to zinc-limitation. This antisense transcript (adh1AS) shows a reciprocal expression pattern to that the adh1 mRNA partner. In this study we show that increased expression of the adh1AS transcript in zinc-limited cells is necessary for the repression of adh1 gene expression and that the increased level of the adh1AS transcript in zinc-limited cells is a result of two mechanisms. At the transcriptional level, the adh1AS transcript is expressed at a high level in zinc-limited cells. In addition to this transcriptional control, adh1AS transcripts preferentially accumulate in zinc-limited cells when the adh1AS transcript is expressed from a constitutive promoter. This secondary mechanism requires the simultaneous expression of adh1. Our studies reveal how multiple mechanisms can synergistically control the ratio of sense to antisense transcripts, and highlight a novel mechanism by which adh1 gene expression can be controlled by cellular zinc availability

2012-12-12 | GSE39701 | GEO

H2A.Z marks antisense promoters and has positive effects on antisense transcript levels in budding yeast

Project description:The histone variant H2A.Z, which has been reported to have both activating and repressive effects on gene expression, is known to occupy nucleosomes at the 5’ ends of protein-coding genes. We now find that H2A.Z is also significantly enriched in gene coding regions and at the 3’ ends of genes in budding yeast, where it co-localises with histone marks associated with active promoters. By comparing H2A.Z binding to global gene expression in budding yeast strains engineered so that normally unstable transcripts are abundant, we show that H2A.Z is required for normal levels of antisense transcripts as well as sense ones. High levels of H2A.Z at antisense promoters are associated with decreased antisense transcript levels when H2A.Z is deleted, indicating that H2A.Z has an activating effect on antisense transcripts. Decreases in antisense transcripts affected by H2A.Z are accompanied by increased levels of paired sense transcripts. Therefore, the effect of H2A.Z on protein coding gene expression is a reflection of its importance for normal levels of both sense and antisense transcripts.

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A natural antisense transcript of notch-1 regulates cell cycle

Project description:A natural antisense transcript of notch-1 regulates cell cycle

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Zinc-dependent regulation of the adh1 antisense transcript in fission yeast

2012-12-12 | E-GEOD-39701 | biostudies-arrayexpress

Transcript Analysis of Human Mammary Cell Subsets

Project description:This SuperSeries is composed of the following subset Series: GSE11387: Transcript Analysis of Human Mammary Cell Subsets using SAGE GSE11394: Transcript Analysis of Human Mammary Cell Subsets by Chip Technology Keywords: SuperSeries Refer to individual Series

2008-05-09 | E-GEOD-11395 | biostudies-arrayexpress

Expression of Mouse and Human Sense-Antisense Transcript

Project description:Increasing numbers of sense–antisense transcripts (SATs), which are transcribed from the same chromosomal location but in opposite directions, have been identified in various eukaryotic species, but the biological meanings of most SATs remain unclear. To improve understanding of natural sense–antisense transcription, we performed comparative expression profiling of SATs conserved among humans and mice. Using custom oligo-arrays loaded with probes that represented SATs with both protein-coding and non-protein–coding transcripts, we showed that 33% of the 291 conserved SATs displayed identical expression patterns in the two species. Among these SATs, expressional balance inversion of sense–antisense genes was mostly observed in testis at a tissue-specific manner. Northern analyses of the individual conserved SAT loci revealed that: (1) a smeary hybridization pattern was present in mice, but not in humans, and (2) small RNAs (about 60 to 80 nt) were detected from the exon-overlapping regions of SAT loci. In addition, further analyses showed marked alteration of sense–antisense expression balance throughout spermatogenesis in testis. These results suggest that conserved SAT loci are rich in potential regulatory roles that will help us understand this new class of transcripts underlying the mammalian genome. Keywords: Expression profile of mouse and human sense-antisense transcript

2008-02-20 | GSE9581 | GEO

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