Project description:RNAseq was performed by to compare gene expression between wildtype and Smchd1 KO ES cells, the gene expression pattern in Dux KO mutants , Double KO mutant Tet-TKO mutants and Tet TKO plus SMCKHD1 KO mutants were analyzed by RNAseq.
Project description:Single cell RNAseq from brain endothelial cells (BECs) isolated from wild-type and Iqgap2-/- (KO) mice to identify transcriptional changes in BECs caused by loss of Iqgap2. Mouse brains were dissociated to single cell suspension and labelled with CD31. CD31+ cells were flow sorted and processed for single cell RNAseq.