Project description:This series compares gene expression in chicken peripheral blood lymphocyte (PBL) derived macrophages following stimulation with either XL10 E. coli or S. typh LPS over an 8 hour time course (0,1,2,4,and 8 hours). For the late time points, the stimuli were removed after 2 hours and fresh media was added for the remaining time. Keywords: time-course
Project description:This series compares gene expression in chicken peripheral blood lymphocyte (PBL) derived macrophages following stimulation with either XL10 E. coli or S. typh LPS over an 8 hour time course (0,1,2,4,and 8 hours). For the late time points, the stimuli were removed after 2 hours and fresh media was added for the remaining time.
Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity Keywords: time-course
Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity Keywords: time-course
Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity Keywords: time-course
Project description:Purpose: The goal of this study is to identify dynamic expression profiles of epigenetic genes in BMDMs induced by LPS for a time-course stimulaiton. Methods: We profiled RNA-seq in bone marrow-derived macrophages stimulated with LPS for 0h, 4h, 8h, 12h, 24h. Results: Epigenetic genes showed distinct expression patterns under LPS stimulation in different times
Project description:We studied the lipidome (236 individual lipid species including sphingolipids, ceramides, cholesterol, gangliosides, phosphatidylethanolamines) in basal and LPS-stimulated human monocyte derived macrophages (MDMs) over a time-course by LC-MS (30min, 3h, 8h, 16h; n=12 human donors). In order to explore how transcriptomic changes induced by LPS stimulation can correlate with changes in the lipidome, we performed RNAsequencing on MDMs from 4 donors over a time-course (30min, 3h, 8h, 16h)
Project description:Purpose: The goal of this study is to identify differential expression profiles between WT and Setd2-KO in BMDMs induced by LPS for a time-course stimulaiton. Methods: We profiled RNA-seq in bone marrow-derived macrophages stimulated with LPS for 0h, 12h, 24h. Results: We find significant up and down regulated genes in Setd2-KO BMDMs under LPS stimulation.
Project description:These microarrays were performed for use in a genome-wide scan for LPS-regulated genes in mouse macrophages, in order to construct a list of LPS-regulated genes for detailed interrogation on custom microarrays (see GSE19490 for custom array analysis). Mouse macrophages (bone marrow-derived macrophages, BMM) were stimulated with the TLR4 agonist, lipopolysaccharide, over a time course (0, 0.5, 2, 6, 24h) and analysed in biological duplicate by commercial Illumina microarray.
