Project description:Brasenia schreberi is a perennial aquatic herb. We determined the complete chloroplast genome sequence for B. schreberi using Illumina sequencing data. The complete chloroplast sequence is 158,993?bp, include large single-copy (LSC) region of 88,779?bp, small single-copy (SSC) region of 21,665?bp, a pair of invert repeats (IR) regions of 21,078?bp. Plastid genome contains 132 genes, 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis base on 12 chloroplast genomes indicates that B. schreberi is sister to Cabomba spp. in Cabombaceae.

| S-EPMC7707297 | biostudies-literature

Structure and ion physiology of Brasenia schreberi glandular trichomes in vivo.

Project description:Brasenia schreberi is a critically endangered aquatic basal angiosperm. In this work, we characterized the structure of the glandular trichomes of B. schreberi morphologically and histochemically. We used a variety of structural, histochemical and permeability stains for the characterization, and we tested the effects of stress in vivo using NaCl and ethanol. We observed that the glandular trichome of B. schreberi are composed of two disk-like stalk cells, and a glandular cell which surround a cuticular storage space. The cuticle is discontinuous at the surface of the shoots. Nearly half of young trichomes senesced in 0.9% NaCl, and mature trichomes senesced at 1.8% NaCl. About half of young trichomes senesced under 3% ethanol and mature trichomes senesced in 2% ethanol after 20 min of treatment. The physiology of glandular trichomes affects the way they secrete mucilage via storage space at a young stage. The trichomes become permeable and absorb ions when mature. This transition depends on the osmiophilic material and the dynamic protoplast. It can accelerate senescence and disassembly by ion accumulation. Permeability tests and ion treatments of glandular trichomes provide new insights for fertilizer research. Our study highlights the structure and physiology of B. schreberi glandular trichomes.

| S-EPMC6802583 | biostudies-literature

Epiphytic bacteria

Project description:16S amplicons obtained from epiphytic genomes isolated from different seeds Raw sequence reads

| PRJNA911736 | ENA

Draft genome of Brasenia schreberi, a worldwide distributed and endangered aquatic plant.

Project description:ObjectivesBrasenia is a monotypic genus in the family of Cabombaceae. The only species, B. schreberi, is a macrophyte distributed worldwide. Because it requires good water quality, it is endangered in China and other countries due to the deterioration of aquatic habitats. The young leaves and stems of B. schreberi are covered by thick mucilage, which has high medical value. As an allelopathic aquatic plant, it can also be used in the management of aquatic weeds. Here, we present its assembled and annotated genome to help shed light on medial and allelopathic substrates and facilitate their conservation.Data descriptionGenomic DNA and RNA extracted from B. schreberi leaf tissues were used for whole genome and RNA sequencing using a Nanopore and/or MGI sequencer. The assembly was 1,055,148,839 bp in length, with 92 contigs and an N50 of 22,379,495 bp. The repetitive elements in the assembly were 555,442,205 bp. A completeness assessment of the assembly with BUSCO and compleasm indicated 88.4 and 90.9% completeness in the Eudicots database and 95.4 and 96.6% completeness in the Embryphyta database. Gene annotation revealed 67,747 genes that coded for 73,344 proteins.

| S-EPMC10913576 | biostudies-literature

Pleurozium schreberi isolate:Blido

Project description:Pleurozium schreberi isolate:Blido Genome sequencing and assembly

| PRJNA509035 | ENA