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Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)

ABSTRACT: Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)

PROVIDER: PRJNA906591 | ENA |

REPOSITORIES: ENA

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Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

Project description:Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

| PRJNA906588 | ENA

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

Project description:Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

| PRJNA906590 | ENA

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)

Project description:we established a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC3D-hIO) within hIO derived from human pluripotent stem cells.

2023-11-20 | GSE219017 | GEO

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

2023-11-20 | GSE219016 | GEO

Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids.

Project description:Three-dimensional human intestinal organoids (hIO) are widely used as a platform for biological and biomedical research. However, reproducibility and challenges for large-scale expansion limit their applicability. Here, we establish a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC3D-hIO) within hIO derived from human pluripotent stem cells. The intrinsic self-organisation property of ISC3D-hIO, combined with air-liquid interface culture in a minimally defined medium, forces ISC3D-hIO to differentiate into the intestinal epithelium with cellular diversity, villus-like structure, and barrier integrity. Notably, ISC3D-hIO is an ideal cell source for gene editing to study ISC biology and transplantation for intestinal diseases. We demonstrate the intestinal epithelium differentiated from ISC3D-hIO as a model system to study severe acute respiratory syndrome coronavirus 2 viral infection. ISC3D-hIO culture technology provides a biological tool for use in regenerative medicine and disease modelling.

| S-EPMC10821882 | biostudies-literature

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-20 | GSE219018 | GEO

Xeno-free and Chemically Defined Human System for Culturing Human Epidermal Keratinocytes

Project description:Xeno-free and Chemically Defined Human System for Culturing Human Epidermal Keratinocytes

| PRJNA431596 | ENA

Transcription profiling of human embryonic stem cells and their differentiated progeny grown in chemically defined conditions

Project description:Comparison of H9 human embryonic stem cells, grown in chemically defined and animal-product free conditions, with their differentiated progeny - mesendodermal progenitors arisen as the result of changes made to chemically defined hESC growth conditions. Contributes to a larger work on the use of chemically defined growth conditions to direct hESC differentiation.

2009-07-31 | E-MEXP-930 | biostudies-arrayexpress

Transcription profling of human embryonic stem cells differentiated into endoderm progenitors in chemically-defined media.

Project description:Human embryonic stem cells differentiated into endoderm progenitors in chemically-defined media.

2010-04-02 | E-MEXP-2373 | biostudies-arrayexpress

Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production.

Project description:The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity. One major hurdle for clinical AAV applications is large-scale manufacturing. In this regard, the baculovirus-based AAV production system is highly attractive due to its scalability and predictable biosafety. Here, we describe a simple method to improve the baculovirus-based AAV production using the ExpiSf Baculovirus Expression System with a chemically defined medium for suspension culture of high-density ExpiSf9 cells. Baculovirus-infected ExpiSf9 cells produced up to 5 × 1011 genome copies of highly purified AAV vectors per 1 mL of suspension culture, which is up to a 19-fold higher yield than the titers we obtained from the conventional Sf9 cell-based system. When mice were administered the same dose of AAV vectors, we saw comparable transduction efficiency and biodistributions between the vectors made in ExpiSf9 and Sf9 cells. Thus, the ExpiSf Baculovirus Expression System would support facile and scalable AAV manufacturing amenable for preclinical and clinical applications.

| S-EPMC7591331 | biostudies-literature

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